Protocols of Actinomycetes Microbiology to Gene editing

1. Isolation of Actinomycetota by different methods
Ramzan Ahmed, Birson Ingti, Noor Afsha, Sirikanjana Thongmee, Yugal Kishore Mohanta

2. Recent-Traditional methods for Isolating Diverse Actinobacteria
Omnia Mohamed Abd allah, Dina Hatem Amin

3. Isolation of actinobacteria from caves and limestone habitats
Pharada Rangseekaew, Wasu Pathom-aree

4. Isolation of endophytic actinobacteria from different sources
Wasu Pathom-aree, Benyapa Kitwetch, Mathurin Meethangdee, Pharada Rangseekaew

5. Sample treatment and Protocols for the cultivation of marine actinomycetes
Hasti Ramavat, Ankita Dobariya and Satya P. Singh

6. A protocol for screening, cultivation, and molecular identification of rare and slow-growing actinomycetes with biosynthetic gene clusters
Bhagwan Narayan Rekadwad, Vikas Balajirao Maske

7. Isolation and Screening of Enzymes from Actinobacteria
Apsara S. Babu, K.V. Bhaskara Rao

8. RSM-Based Media Optimization for Enzyme Production By Streptomyces
Pavithra M, Mohamed Hasan M, Sai Ramesh A

9. Protocols for the Screening, Extraction, and Purification of the Hydrolytic Enzymes from Marine Actinomycetes
Ankita J. Dobariya, Hina N. Radadiya, Gira P. Mankad, Ramesh K. Kothari, Satya P. Singh

10. Marine Actinomycetes' Hydrolytic Enzymes: A structural characterization.
Sai Ramesh A, Rajamathanghi R, Lavanya Balaji

11. Isolation of Beta-Glucanase Encoding Genes from Actinobacterial Genus Streptomyces Spp. and its Overexpression in E. coli Heterologous System
Lekshmi K. Edison, N. S. Pradeep

12. Isolation and Screening of Enzyme Inhibitors from Marine Actinobacteria
Apsara S. Babu, K.V. Bhaskara Rao

13. Screening of amylase and lipase enzymes inhibitors from Actinomycetota
Ramzan Ahmed, Shiela Chetri, Noor Afsha, Sirikanjana Thongmee

14. Screening and Characterization of Carotinoids Pigment from the Actinobacteria
Jayachandra S. Yaradoddi, Chetan D. M, Gangadhar Megeri

15. Screening of Biosurfactant activity of Actinobacteria
Jaysree RC

16. Traditional Screening of Actinomycetes for Antibacterial, Antifungal and Antiviral Properties
Sai Ramesh A, Mohamed Hasan M, Kiran Sharma B, Pavithra M

17. Isolation and screening of actinobacteria with antibacterial activity and its production through shake flask culture method
Durairaj Thirumurugan, Ramasamy Vijayakumar

18. Screening of Antimycobacterial property of actinobacteria
Muniyan Rajiniraja

19. Screening actinomycetes for novel antimycobacterial and anti-cancer molecules.
Balaji Muralikrishnan, Vipin Mohan Dan

20. In vitro antioxidant protocols for Actinobacteria
Charles Lekhya Priya

21. Protocol for Antileishmanial activity using marine Actinobacteria
Veena Sreedharan, Karthik Loganathan, K V Bhaskara Rao

22. Shining a Light on the Potent Anti-Malarial Activity actinomycete extract: A Comprehensive In Vitro and In Vivo Evaluation
A. Vishnu Kirthi, L. Karthik, Tarun Keswani, Anirban Sengupta, Shilpi Garg

23. Screening of marine actinobacteria for postprandial anti-diabetic activity In-vitro and In -vivo Methods
Sathish Kumar SR

24. Exploring the Larvicidal Activity of actinomycetes Extracts
A. Vishnu Kirthi, L. Karthik, Tarun Keswani, Anirban Sengupta, Shilpi Garg, Chitrakshi Consul

25. Evaluation of Actinobacteria's antibiofilm properties using qualitative and quantitative methods
Nisha, Gaurav Pant, Gaurav Kumar

26. Microplastic degradation by Actinobacteria
Uttra Chauhan, Gaurav Kumar, Gaurav Pant

27. Antibacterial and Antibiofilm activity of biosynthesized nanoparticles from Actinomycetes: An alternative, environmental-free, sustainable approach
Anand Ravichandran, Abhitha Venkataramakrishnan, Ayyadurai Niraikulm, Saravanan Periasamy

28. Extraction and purification of rapamycin from Streptomyces hygroscopicus
Sanjeev K Ganesh, Mohanasrinivasan V, Subathra Devi C

29. Isolation and Identification of Squalene from the ethyl acetate extract of Streptomyces sp. VITGV156 (MCC 4965)
Veilumuthu Pattaulavar, Ramamoorthy Siva, Jayaraj Rajesh Banu, Sasikumar Sundarasan, John Godwin Christopher

30. Docking and ADME studies
K. Prem Anand, K. Suthindhiran

31. Methods and protocols for the extraction of the genomic DNA, gene cloning, and heterologous expression from the actinomycetes of extreme habitats
Hasti Ramavat, Satya P. Singh

32. Activation of silent genes in actinomycetes by different methods
Veilumuthu Pattaulavar, Ramamoorthy Siva, John Godwin Christopher

33. Multimodal Spectroscopy Based Proteomics and Metabolomics Approach to Explore Actinomycetes
Akhiya A R, Vartika Lohani, Soumen Kundu, Md Quasid Akhter, Balendu Upmanyu, Swarnendu Bag

34. Isolation of DNA and RNA from actinobacteria
Shree Kumari GR, Ranjitha Dhevi V. Sundar, Ajith Kumar K, Lokesh Ravi

35. Extraction of DNA from Actinomycetota and amplification of the 16S rRNA gene
Manik Prabhu Narsing Rao, Arinthip Thamchaipenet

36. Optimization of colony PCR for the identification of actinobacteria by sequencing of the 16S rDNA
Mustafa Oskay

37. Identification of actinomycetes by polyphasic taxonomy
Balaji Muralikrishnan

38. Phylogenetic Analysis of Actinomycetes
Yashaswini D M, Andrea Michelle, Monisha U, Lokesh Ravi

39. Genetic profiling of actinobacterial genomes through polymerase chain reaction based techniques
Bhatele Pranita, Khanna Asha

40. In Silico Analysis of Phylogenetic Relationship and Non-synonymous Polymorphisms in Actinobacteria
K. Swetha, Shasmeen Shora P. Chandra Sekar, Ipsita Debnath, M. Joyce Nirmala, R. Nagarajan

41. Isolation of metagenomic DNA and data analysis
Shabiha Nudrat Hazarika, Kangkon Saikia, Debajit Thakur

42. Practical Methodology of Illumina Sequencing Investigating Actinobacteria Genomes
Alaa F. Elsayed, Dina H. Amin

43. Digital DNA-DNA hybridization for the identification of Actinomycetota species
Manik Prabhu Narsing Rao, Awalagaway Dhulappa

44. antiSMASH: Genome-mining and genome-wide analysis of gene clusters in Actinopolyspora species
Bhagwan Narayan Rekadwad, Kasbe Prajapati Sudhakar

45. Transcriptome Analysis of Actinomycetes RNA Sequencing data: A bioinformatics protocol for beginners
Sankaranarayanan Gomathinayagam, Srivarshini Sankar, Nalini Easwaran, Loganathan Karthik, Kodiveri Muthukaliannan Gothandam

46. Single-cell RNA sequencing of the actinomycetes
Akshatha Prasanna, Aishwarya Das, T S Sai Pawan

47. Preparation of Streptomyces RNA for transcriptomics
Balaji Muralikrishnan

48. A protocol for CRISPR-Cas9 mediated knockout construction in Actinobacteria
Khushal Khambhati, Vijai Singh

49. Design of gRNA for DNA-directed RNA polymerase beta subunit (rpoB) gene in Actinomycete
V. Naveen Kumar

50. Acute and Chronic Toxicity of Bioactive Compounds from Actinobacteria
Joyce Nirmala M, Uma Kizhuveetil, Ipsita Debnath, Ramamurthy Nagarajan

51. Production, extraction, purification and characterization of bioactive derivatives of phthalate ester from Marine Streptomyces sp.VITJS4
C.Subathra Devi, Jemimah Naine.S, Maneesha M, Sanjeev K Ganesh, Kothari Neeti, Mohanasrinivasan .V

Biography

L. Karthik has an extensive expertise in Actinobacteria, Synthetic biology and Drug Discovery Strategies and is presently working as a Project Leader, Synthetic Biology at Salem Microbes Pvt. Ltd, Salem, India. He is Top 2% of all scientists in the world (the Stanford University report published by Elsevier on 10th October 2022&2023).

He is an elected Fellow member of Linnean Society of London. He did first postdoctoral training at the Shanghai Jiao Tong University, China under the guidance of eminent scientist Prof. Zhiyong Li. Further he has completed his second postdoctoral fellowship in ECUST, Shanghai, China under the guidance of eminent scientist Prof. Lixin Zhang. He received his Ph.D. degree in Microbiology from the VIT University in 2013. He has published around 75 research papers (h index-26), 5 Books and 6 patents and 6 products commercialized.

He is a communication committee member of International Chemical Biology Society, USA and President of Society of Chemical and Synthetic biology, India. In 2016, he got Prestigious “Young Scientist Award” from Indian Association of Applied Microbiologists for his outstanding research work. and got Best Research scholar award-2012 from VIT University. He got 200 young scientist-2012 fellowship (DST) to attend international conference. He got Best VIT ALUMNI AWARD-2022 from VIT University, TN, India. He is Associate Editor of Frontiers in Microbiology and Review editor of Frontiers in Marine Science and also Associate Editor of Current Chinese Engineering Science Journal (Bentham Science). Recently, he proved COVID-19 activity of Kabasura Kudineer (Siddha Herbal formulation). During COVID-19 wave, this drug saved lot of life. For this contribution, he got COVID warrior award from SEED Trust, TN, India.