Quantitation and Mass Spectrometric Data of Drugs and Isotopically Labeled Analogs: 1st Edition (Hardback) book cover

Quantitation and Mass Spectrometric Data of Drugs and Isotopically Labeled Analogs

1st Edition

By Ray H. Liu, Dennis V. Canfield, Sheng-Meng Wang

CRC Press

510 pages | 81 B/W Illus.

Purchasing Options:$ = USD
Hardback: 9781420094978
pub: 2009-08-05
SAVE ~$39.75
$265.00
$225.25
x
eBook (VitalSource) : 9780429250552
pub: 2009-08-05
from $132.50


FREE Standard Shipping!

Description

The analysis of drugs and their metabolites in biological media are now expected to routinely achieve ± 20% accuracy in the ng/mL concentration level. Therefore, the availability and the selection of quality ion-pairs designating the analytes and their isotopically labeled analogs (ILAs) are important considerations in achieving the accuracy of quantitation results. Assisting scientists with this process, Quantitation and Mass Spectrometric Data of Drugs and Isotopically Labeled Analogs provides an extremely valuable reference for labs involved in the analysis of therapeutic and abused drugs.

Part One of this comprehensive volume illustrates approaches, mechanisms, and challenges pertaining to the use of isotopic analogs as internal standards for drug quantitation. The second section is a systematic compilation of full-scan mass spectra of drugs and their analogs, as parent compounds and as derivatives resulting from various chemical derivatization approaches, commonly encountered in today’s labs. Based on the mass spectra data presented in the second section, Part Three provides corresponding tables of ion-pairs which can potentially be adapted to designate the drugs and their isotopic analogs in the analytical processes. Relative quality of these ion-pairs (cross-contribution to the intensity of these ions by their isotopic analogs) is included in these tables.

With more than 1500 full-scan mass spectra and quick access data tables, this text represents the authors’ years of work compiling mass spectra of the many chemical derivatization forms of drugs, their metabolites, and their isotopically labeled counterparts. The unparalleled scope of this compilation makes it a critical one-stop reference for those involved in drug analyses of biological specimens and interpretation of results.

 

Reviews

The information presented in this book is definitely of value to any laboratory engaged in toxicology analysis and the quantification of drugs.

—Maria Reid, Royal Canadian Mounted Police, Canadian Society of Forensic Science, Vol. 43, No. 1, March 2010

The book is of obvious utility to those analysts who work in

drug analysis and to those who are confronted occasionally with

a drug-analysis problem. The strategies outlined in this work are

important to anyone who is conducting trace analysis by

chromatography coupled with mass spectrometry.

—Michael L. Gross, Journal of the American Society for Mass Spectrometry

Table of Contents

Part One. Isotopically Labeled Analog as Internal Standard for Drug Quantitation — Methodology

Quantitation of Drug in Biological Specimen — Isotopically Labeled Analog of the Analyte as Internal Standard

Introduction

Significance of Accurate Quantitation

Preferred Calibration Method

Internal Standard and Quantitation Ions

Inadequate Isotopic Purity — An Extrinsic Factor

Cross-Contribution Derived from Ion Fragmentation Mechanism — An Intrinsic Factor

Fitting Calibration Data

2H- Versus 13C-Analogs as Internal Standards

Concluding Remarks

References

Isotopically Labeled Analog of the Analyte as Internal Standard for Drug Quantitation — Chemical Derivatization and Data Collection and Evaluation

Introduction

Chemical Derivatization

Production of Most Favorable Ion-Pairs for Drug Quantitation

Exemplar Studies

Isotopically Labeled Analogs and Chemical Derivatization Groups

Ion Intensity Cross-Contribution Data

Full-Scan Mass Spectra

Selected Ion Monitoring and Calculation of Cross-Contribution Data:

Direct Measurement, Normalized Direct Measurement, Internal Standard Method, Standard Addition Method

Assessing the Accuracy of Empirically Determined Cross-Contribution Data:

Experimentally Observed Concentration, Theoretically Calculated Concentration, Comparing Empirically Derived and Theoretically Calculated Concentrations — Graphic Presentation, Summary

Compilation of Full-Scan Mass Spectra and Ion Intensity Cross-Contribution Tables:

Derivatization Procedures, Instrumentation, and Analytical Parameters

Collection of Mass Spectrometric Data

Ion Intensity Cross-Contribution Data

Concluding Remarks

References

Part Two. Mass Spectra of Commonly Abused Drugs and Their Isotopically Labelled Analogs in Various Derivatization Forms

Table of Contents for Appendix One

Stimulants

Opioids

Hallucinogens

Depressants/Hypnotics

Antianxiety Agents

Antidepressants

Others

Part Three. Cross-Contributions of Ion Intensity Between Analytes and Their Isotopically Labeled Analogs in Various Derivatization Forms

Table of Contents for Appendix Two

Stimulants

Opioids

Hallucinogens

Depressants/Hypnotics

Antianxiety Agents

Antidepressants

Others

Index

About the Authors

Author

Ray H. Liu is a professor in the Department of Medical Technology at Fooyin University in Taiwan and professor emeritus in the Department of Justice Sciences at the University of Alabama in Birmingham. Sheng-Meng Wang is a professor of forensic science and director of scientific laboratories at Central Police University in Taiwan. Dennis V. Canfield is the manager of the Bioaeronautical Sciences Research Laboratory at the U.S. Federal Aviation Administration’s Civil Aerospace Medical Institute (CAMI) in Oklahoma City, Oklahoma.

Subject Categories

BISAC Subject Codes/Headings:
LAW041000
LAW / Forensic Science
MED096000
MEDICAL / Toxicology
SCI013000
SCIENCE / Chemistry / General
SCI013010
SCIENCE / Chemistry / Analytic