1st Edition
Quantitive Analysis Of Biospec
244 Pages
by
Routledge
244 Pages
by
Routledge
Also available as eBook on:
First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement... Read more
1 Electrophoretic Analysis of Reversible Interactions 2 Determination of Affinity Constants by Capillary Electrophoresis 3 Measurement of Binding Constants by Frontal Affinity Chromatography 4 Determination of Affinity Constants by Partition Equilibrium Methods 5 Determination of Transmembrane Protein Affinities for Solutes by Frontal Chromatography 6 Modeling of Chiral Liquid Chromatography using an Immobilized Protein as the Selector 7 Determination of Receptor-Ligand Affinity Constants 8 Determination of Equilibrium and Kinetic Constants for the Interaction of GTPases with Nucleotides, Regulators and Effectors 9 Biospecific Interaction Analysis using Integrated Optics Techniques 10 Receptor Binding Studies on Lipid Vesicles using the BIAcore 11 The Analysis of Surface Plasmon Resonance-based Biosensor Data using Numerical Integration: The Epidermal Growth Factor Receptor-Ligand Interaction as an Example 12 Affinity Chromatography for the Determination of Interactions between Drugs and Proteins: HPLC and CE Methods Employing ‘Secondary and Dynamic’ Equilibria
Biography
Per Lundahl, Andreas Lundqvist, Eva Greijer- all Department of Biochemistry, Biomedical Center, Uppsala University, Uppsala, Sweden
