2nd Edition

RNA Nanotechnology and Therapeutics

Edited By Peixuan Guo, Kirill A. Afonin Copyright 2022
    644 Pages 112 Color & 44 B/W Illustrations
    by CRC Press

    644 Pages 112 Color & 44 B/W Illustrations
    by CRC Press

    Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, immunorecognition of RNA nanomaterials, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.

    • Presents the latest research and discoveries in RNA nanotechnology
    • Features contributions from world-class experts in the field
    • Covers RNA nanoparticles in therapeutics
    • Describes self-assembled RNA nanoparticles

    Part I: Concepts and Definitions in RNA Nanotechnology
    Co-edited by Peixuan Guo and Kirill A. Afonin
    1. RNA Structure and Folding
    Li, X., Guo, P.
    2. RNA Regulation and Function in Nature
    Li, Z., Binzel, D., Guo, P.
    3. Principles and Fundamentals of RNA Nanotechnology
    Zhang, L., Guo, P.
    4. Computation and Folding Predictions
    Du, L., Liang, C., Guo, P.
    5. Enzymatic Synthesis and Modification of RNA Nanoparticles
    Binzel, D., Guo, P.
    6. Synthetic and Enzymatic Methods for RNA Labeling and Modifications 
    Satheesh Ellipilli and Peixuan Guo
    7. Methods and Assembly of RNA Nanotechnology
    Hongzhi Wang, Chenxi Liang, Peixuan Guo
    8. Purification, Characterization, and Structure Determination of RNA Nanoparticles
    Satheesh Ellipilli, Nicolas D. Burns, and Peixuan Guo
    9. SELEX
    Liang, C., Guo, P.
    10. Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology
    Guo, P., Haque, F., Hallahan, B., Reif, R., Li, H.
    Part II: Design, Synthesis, and Characterization Methods in RNA Nanotechnology
    Co-edited by Peixuan Guo and Kirill A. Afonin
    11. The Natural Versatility of RNA
    Rolband, L., Shevchenko, O., West, C., Conway, C., Striplin, C., Afonin, K.
    12. Nucleic Acids as a building material in nanotechnology
    Halman, J., Afonin, K.
    13. Multiple functionalities for RNA Nanoparticles
    Avila, Y., Panigaj, M.
    14. From Computational RNA Structure Predication to the Design of Biologically Active RNA-based Nanostructures
    Kasprzak, W.K., Shapiro, B.
    15. Application of RNA tertiary structure prediction tool iFoldRNA in RNA nanotechnology
    Wang, J., Dokholyan, N.
    16. HyperFold: A Web Server for Predicating Nucleic Acid Complexes
    Bindewald, E., Shapiro, B.
    17. RNA Switches: Towards Conditional Dynamic RNA-based Constructs for Therapeutics and Bioassays
    Zakrevsky, P., Shapiro, B.
    18. RNA Multiway Junction Motifs as Lego for Construction of Multifunctional RNA Nanoparticles
    Haque, F., Li, X., Guo, P.
    19. Fabrication Methods for RNA Nanoparticle Assembly Based on Bacteriophage Phi29 PRNA Structural Features
    Shu, Y., Wang, H., Seremi, B., Guo, P.
    20. Purification of RNA, modified oligos and RNA nanoparticles
    Hill, J., Vieweger, M., Pi, F., Guo, P
    21. Physicochemical Characterization of Nucleic Acid Nanoparticles
    Miller, D., Khisamutdinov, E.
    22. Light Scattering Techniques for Characterization of NANPs and Their Formulations
    Rolband, L., Krueger, J.
    23. Electron Microscopy of Nucleic Acid Nanoparticles
    Beasock, D., Afonin, K.
    24. A Single FRET Approach for Investigating the Binding Mechanism of Anti-viral Aptamers
    Sen, S., Holmstrom, E.
    25. Entropy and Enthalpy in RNA Nanoparticle Assembly and Thermodynamic Stability for Medical Application Using RNA Nanotechnology
    Binzel, D., Li, X., Guo, P.
    Part III: Immunorecognition of RNA Nanoparticles
    Co-edited by Peixuan Guo and Kirill A. Afonin
    26. Immunorecognition of nucleic acid nanoparticles
    McMillan, J., Johnson, M.
    27. Viral noncoding RNAs in modulating cellular defense and their potential for RNA nanotechnology
    Panigaj, M., Dobrovolskaia, M., Afonin, K.
    28. RIG-I as a therapeutic target for Nucleic Acid Nanoparticles (NANPs)
    Johnson, M.
    29. Driving Dynamic Functions with Programmable RNA Nanostructures
    Chandler, M., Elasmar, D., Eisen, S., Schmedake, L., Afonin, K.
    30. Use of human peripheral blood mononuclear cells to define immunological properties of nucleic acid nanoparticles
    Dobrovolskaia, M., Afonin, K.A.
    Part IV: Delivery of Functional RNA Nanoparticles
    Co-edited by Peixuan Guo, Kirill A. Afonin, and Xiaoting Zhang
    31. The emerging field of RNA Nanotechnology
    Guo, P.
    32. Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics
    Shu, D., Shu, Y., Haque, F., Abdelmawla, S., Guo, P.
    33. RNAi Nanotherapeutics for Localized Cancer Therapy
    Puri, A., Shapiro, B.
    34. Delivery of RNA nanoparticles
    Ke, W., Kozlov, S., Afonin, K.
    35. Recommendations for Planning in vivo Studies for RNAi Therapeutics
    Parlea, L., Shapiro, B.
    36. Mesoporous silica nanoparticles for efficient siRNA delivery
    Tarannum, M., Vivero-Escoto, J.
    37. Method of Large-scale Exosome Purification and Its Use for Pharmaceutical Applications
    Pi, F., Fang, Y., Li, D., Guo, P.
    38. Engineered extracellular vesicle based therapeutics for liver cancer
    Ishiguro, K., Patel, T.
    39. Extracellular Vesicles: An innovative approach to engineering nucleic acid delivery
    Nordmeier, S., Hsiung, F., Portnoy, V.
    40. Extracellular Vesicles (EVs): Naturally Occurring Vehicles for RNA Nanotherapeutics
    Braga, A., Manferrari, G., Smith, J., Pluchino, S.
    41. Harnessing exosomes and bioinspired exosome-like nanoparticles for siRNA delivery
    Lu, M., Huang, Y. 
    Part V: Application and Exploitation in RNA Nanotechnology
    Co-edited by Peixuan Guo, Kirill Afonin, and Yuanyu Huang
    42. RNA structural modeling for therapeutic applications
    Cheng, Y., Zhang, D., Hurst, T., Zou, X., Giangrande, P., Chen, S.
    43. RNA micelles for therapeutics delivery and cancer therapy
    Yin, H., Shu, Y., Guo, P.
    44. Bacteriophage RNA Leading the Way in RNA Nanotechnology for Targeted Cancer Therapy
    Haque, F., Hu, H., Guo, P.
    45. Current State in development of RNAi self-assembled nanostructures
    Ben, M., Salah, H., Rossi, J.
    46. RNA-Based Devices for Diagnostic and Biosensing
    Chandler, M., Afonin, A.
    47. MicroRNAs: Biology and Role in RNA Nanotechnology
    Guo, B., Liu, J., Binzel, D.
    48. Conjugation of RNA aptamer to RNA nanoparticles for targeted drug delivery
    Leonard, M., Pi, F., Guo, P., Zhang, X.
    49. MicroRNAs in human cancers and therapeutic applications
    Yeh, M., Oh, C., Lee, T.
    50. Tuning the Size, Shape and Structure of RNA Nanoparticles for Favorable Cancer Targeting and Immunostimulation
    Guo, S., Xu, C., Yin, H., Hill, J., Pi, F., Guo, P.
    51. Application of RNA nanotechnology in Exosome for gene therapy
    Li, Z., Pi, F., Guo, P.
    52. Application of RNA Aptamers in Nanotechnology and Therapeutics
    Shi, H.


    Peixuan Guo is the Director of the NIH Nanomedicine Development Center at the University of Cincinnati, Ohio and the Director of the NIH/NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy at Purdue University, Indiana. He is also a Fellow of the National Academy of Inventors.

    Kirill Afonin is the Professor of Chemistry at University of North Carolina at Charlotte. He currently serves as the Founding Council Member and Vice President of International Society of RNA Nanotechnology and Nanomedicine (isrnn.org) and is recipient of several prestigious awards among which is Maximizing Investigators' Research Award (MIRA R35) from NIH.

    "This comprehensive, state-of-the-art reference book, edited by experts in the field of nanomedicine, describes the latest research development and challenges in the biophysical and single-molecule approaches in RNA nanotechnology. The book provides interesting coverage on RNA nanoparticles in therapeutics and details self-assembled RNA nanoparticles."

    -Omer Iqbal, MD, FACC, FESC, Doody Review