RNA Nanotechnology and Therapeutics  book cover
2nd Edition

RNA Nanotechnology and Therapeutics

ISBN 9781138312869
Published July 18, 2022 by CRC Press
644 Pages 112 Color & 44 B/W Illustrations

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Book Description

Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, immunorecognition of RNA nanomaterials, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.

  • Presents the latest research and discoveries in RNA nanotechnology
  • Features contributions from world-class experts in the field
  • Covers RNA nanoparticles in therapeutics
  • Describes self-assembled RNA nanoparticles

Table of Contents

Part I: Concepts and Definitions in RNA Nanotechnology
Co-edited by Peixuan Guo and Kirill A. Afonin
1. RNA Structure and Folding
Li, X., Guo, P.
2. RNA Regulation and Function in Nature
Li, Z., Binzel, D., Guo, P.
3. Principles and Fundamentals of RNA Nanotechnology
Zhang, L., Guo, P.
4. Computation and Folding Predictions
Du, L., Liang, C., Guo, P.
5. Enzymatic Synthesis and Modification of RNA Nanoparticles
Binzel, D., Guo, P.
6. Synthetic and Enzymatic Methods for RNA Labeling and Modifications 
Satheesh Ellipilli and Peixuan Guo
7. Methods and Assembly of RNA Nanotechnology
Hongzhi Wang, Chenxi Liang, Peixuan Guo
8. Purification, Characterization, and Structure Determination of RNA Nanoparticles
Satheesh Ellipilli, Nicolas D. Burns, and Peixuan Guo
Liang, C., Guo, P.
10. Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology
Guo, P., Haque, F., Hallahan, B., Reif, R., Li, H.
Part II: Design, Synthesis, and Characterization Methods in RNA Nanotechnology
Co-edited by Peixuan Guo and Kirill A. Afonin
11. The Natural Versatility of RNA
Rolband, L., Shevchenko, O., West, C., Conway, C., Striplin, C., Afonin, K.
12. Nucleic Acids as a building material in nanotechnology
Halman, J., Afonin, K.
13. Multiple functionalities for RNA Nanoparticles
Avila, Y., Panigaj, M.
14. From Computational RNA Structure Predication to the Design of Biologically Active RNA-based Nanostructures
Kasprzak, W.K., Shapiro, B.
15. Application of RNA tertiary structure prediction tool iFoldRNA in RNA nanotechnology
Wang, J., Dokholyan, N.
16. HyperFold: A Web Server for Predicating Nucleic Acid Complexes
Bindewald, E., Shapiro, B.
17. RNA Switches: Towards Conditional Dynamic RNA-based Constructs for Therapeutics and Bioassays
Zakrevsky, P., Shapiro, B.
18. RNA Multiway Junction Motifs as Lego for Construction of Multifunctional RNA Nanoparticles
Haque, F., Li, X., Guo, P.
19. Fabrication Methods for RNA Nanoparticle Assembly Based on Bacteriophage Phi29 PRNA Structural Features
Shu, Y., Wang, H., Seremi, B., Guo, P.
20. Purification of RNA, modified oligos and RNA nanoparticles
Hill, J., Vieweger, M., Pi, F., Guo, P
21. Physicochemical Characterization of Nucleic Acid Nanoparticles
Miller, D., Khisamutdinov, E.
22. Light Scattering Techniques for Characterization of NANPs and Their Formulations
Rolband, L., Krueger, J.
23. Electron Microscopy of Nucleic Acid Nanoparticles
Beasock, D., Afonin, K.
24. A Single FRET Approach for Investigating the Binding Mechanism of Anti-viral Aptamers
Sen, S., Holmstrom, E.
25. Entropy and Enthalpy in RNA Nanoparticle Assembly and Thermodynamic Stability for Medical Application Using RNA Nanotechnology
Binzel, D., Li, X., Guo, P.
Part III: Immunorecognition of RNA Nanoparticles
Co-edited by Peixuan Guo and Kirill A. Afonin
26. Immunorecognition of nucleic acid nanoparticles
McMillan, J., Johnson, M.
27. Viral noncoding RNAs in modulating cellular defense and their potential for RNA nanotechnology
Panigaj, M., Dobrovolskaia, M., Afonin, K.
28. RIG-I as a therapeutic target for Nucleic Acid Nanoparticles (NANPs)
Johnson, M.
29. Driving Dynamic Functions with Programmable RNA Nanostructures
Chandler, M., Elasmar, D., Eisen, S., Schmedake, L., Afonin, K.
30. Use of human peripheral blood mononuclear cells to define immunological properties of nucleic acid nanoparticles
Dobrovolskaia, M., Afonin, K.A.
Part IV: Delivery of Functional RNA Nanoparticles
Co-edited by Peixuan Guo, Kirill A. Afonin, and Xiaoting Zhang
31. The emerging field of RNA Nanotechnology
Guo, P.
32. Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics
Shu, D., Shu, Y., Haque, F., Abdelmawla, S., Guo, P.
33. RNAi Nanotherapeutics for Localized Cancer Therapy
Puri, A., Shapiro, B.
34. Delivery of RNA nanoparticles
Ke, W., Kozlov, S., Afonin, K.
35. Recommendations for Planning in vivo Studies for RNAi Therapeutics
Parlea, L., Shapiro, B.
36. Mesoporous silica nanoparticles for efficient siRNA delivery
Tarannum, M., Vivero-Escoto, J.
37. Method of Large-scale Exosome Purification and Its Use for Pharmaceutical Applications
Pi, F., Fang, Y., Li, D., Guo, P.
38. Engineered extracellular vesicle based therapeutics for liver cancer
Ishiguro, K., Patel, T.
39. Extracellular Vesicles: An innovative approach to engineering nucleic acid delivery
Nordmeier, S., Hsiung, F., Portnoy, V.
40. Extracellular Vesicles (EVs): Naturally Occurring Vehicles for RNA Nanotherapeutics
Braga, A., Manferrari, G., Smith, J., Pluchino, S.
41. Harnessing exosomes and bioinspired exosome-like nanoparticles for siRNA delivery
Lu, M., Huang, Y. 
Part V: Application and Exploitation in RNA Nanotechnology
Co-edited by Peixuan Guo, Kirill Afonin, and Yuanyu Huang
42. RNA structural modeling for therapeutic applications
Cheng, Y., Zhang, D., Hurst, T., Zou, X., Giangrande, P., Chen, S.
43. RNA micelles for therapeutics delivery and cancer therapy
Yin, H., Shu, Y., Guo, P.
44. Bacteriophage RNA Leading the Way in RNA Nanotechnology for Targeted Cancer Therapy
Haque, F., Hu, H., Guo, P.
45. Current State in development of RNAi self-assembled nanostructures
Ben, M., Salah, H., Rossi, J.
46. RNA-Based Devices for Diagnostic and Biosensing
Chandler, M., Afonin, A.
47. MicroRNAs: Biology and Role in RNA Nanotechnology
Guo, B., Liu, J., Binzel, D.
48. Conjugation of RNA aptamer to RNA nanoparticles for targeted drug delivery
Leonard, M., Pi, F., Guo, P., Zhang, X.
49. MicroRNAs in human cancers and therapeutic applications
Yeh, M., Oh, C., Lee, T.
50. Tuning the Size, Shape and Structure of RNA Nanoparticles for Favorable Cancer Targeting and Immunostimulation
Guo, S., Xu, C., Yin, H., Hill, J., Pi, F., Guo, P.
51. Application of RNA nanotechnology in Exosome for gene therapy
Li, Z., Pi, F., Guo, P.
52. Application of RNA Aptamers in Nanotechnology and Therapeutics
Shi, H.

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Peixuan Guo is the Director of the NIH Nanomedicine Development Center at the University of Cincinnati, Ohio and the Director of the NIH/NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy at Purdue University, Indiana.

Kirill Afonin is the Professor of Chemistry at University of North Carolina at Charlotte. He currently serves as the Founding Council Member and Vice President of International Society of RNA Nanotechnology and Nanomedicine (isrnn.org) and is recipient of several prestigious awards among which is Maximizing Investigators' Research Award (MIRA R35) from NIH.