1st Edition

Recombinant DNA Principles and Methodologies

Edited By

James Greene



ISBN 9780824799892
Published June 25, 1998 by CRC Press
768 Pages

USD $400.00

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Book Description

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

  • plasmid biology
  • nucleic acid biochemistry
  • restriction enzymes
  • cloning strategies
  • gel electrophoresis
  • southern and northern blotting
  • preparation of probes
  • phage lambda biology
  • cosmids and genome analysis
  • cloned gene expression
  • polymerase chain reaction
  • conventional and automated DNA sequencing
  • site-directed mutagenesis
  • and more!
    Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
  • Table of Contents

    Why Cloning?, James J. Greene
    Biochemistry of Nucleic Acids, Paul S. Miller
    Enzymology of Recombinant DNA, Frank J. Castora
    Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges
    Formulation of Cloning Strategies, John B. Dame
    Isolation and Purification of Nucleic Acids, James J. Greene
    Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora
    Hybridization Probes, Frank J. Castora and James J. Greene
    cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga
    Molecular Cloning by Plasmid Vectors, Paulina Balbás and Francisco Bolívar
    Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss
    Cosmid Cloning, John Collins and Werner Lindenmaier
    Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short
    Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver
    Approaches to the Expression of Foreign Genes, Allan R. Shatzman
    Screening for Specific Recombinant Clones, Lucia Irene González-Villaseñor and Mark M. Manak
    Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach
    DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao
    Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz

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    Reviews

    "
    …provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits.
    "- Trends in Genetics