Recombinant DNA Principles and Methodologies: 1st Edition (Hardback) book cover

Recombinant DNA Principles and Methodologies

1st Edition

Edited by James Greene

CRC Press

768 pages

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Hardback: 9780824799892
pub: 1998-06-25
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This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.

Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

  • plasmid biology

  • nucleic acid biochemistry

  • restriction enzymes

  • cloning strategies

  • gel electrophoresis

  • southern and northern blotting

  • preparation of probes

  • phage lambda biology

  • cosmids and genome analysis

  • cloned gene expression

  • polymerase chain reaction

  • conventional and automated DNA sequencing

  • site-directed mutagenesis

  • and more!

    Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

  • Reviews


    …provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits.

    "- Trends in Genetics

    Table of Contents

    Why Cloning?, James J. Greene

    Biochemistry of Nucleic Acids, Paul S. Miller

    Enzymology of Recombinant DNA, Frank J. Castora

    Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges

    Formulation of Cloning Strategies, John B. Dame

    Isolation and Purification of Nucleic Acids, James J. Greene

    Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora

    Hybridization Probes, Frank J. Castora and James J. Greene

    cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga

    Molecular Cloning by Plasmid Vectors, Paulina Balbás and Francisco Bolívar

    Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss

    Cosmid Cloning, John Collins and Werner Lindenmaier

    Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short

    Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver

    Approaches to the Expression of Foreign Genes, Allan R. Shatzman

    Screening for Specific Recombinant Clones, Lucia Irene González-Villaseñor and Mark M. Manak

    Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach

    DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao

    Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz

    Subject Categories

    BISAC Subject Codes/Headings:
    SCIENCE / Life Sciences / Cytology
    SCIENCE / Life Sciences / Biology / Molecular Biology