1st Edition

Regulation of Gene Expression by Small RNAs

Edited By Rajesh K. Gaur, John J. Rossi Copyright 2009
    460 Pages 4 Color & 72 B/W Illustrations
    by CRC Press

    458 Pages 4 Color & 72 B/W Illustrations
    by CRC Press

    New Findings Revolutionize Concepts of Gene Function

    Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it’s been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression.

    Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting current and future researchers, this unique groundbreaking work —

    • Provides a suite of cutting-edge resources for the study of microRNA ontology and function
    • Includes a technology guide for those seeking to assay microRNA expression
    • Explores the mechanisms by which microRNAs regulate gene expression in animal cells, including the regulation of gene expression by RNA-mediated transcriptional gene silencing
    • Discusses a fast and low-cost approach for reversing genetic influences in mammals
    • Looks at breakthroughs in the use of microRNA-based therapy for HIV and cancer

    This volume captures the essence of the breadth and excitement surrounding the newly discovered regulatory roles of small RNAs. The powerful new approach in the study of gene function described in this text is leading to some remarkable findings that have the potential to revolutionize our understanding of genetic function and the treatment of diseases otherwise considered intractable.

    MicroMining: Computational Approaches to microRNA Discovery, A. Grundhoff

    Animal microRNA Gene Prediction, O. Snøve and P. Sætrom

    A Suite of Resources for the Study of microRNA Ontology and Function, P. Sethupathy, M. Megraw, and A. G. Hatzigeorgiou 

    Regulation of Translation and mRNA Stability by Hfq-Binding Small RNAs in Escherichia coli, H. Aiba

    Mechanisms by which microRNAs Regulate Gene Expression in Animal Cells, Y. Yu and T. W. Nilsen

    The microRNAs of Caenorhabditis elegans, M. J. Nolde and F. J. Slack

    Isolation and Characterization of Small RNAs in Caenorhabditis elegans, C. Ushida and Y. Hokii

    MicroRNA Tales in Fly Development, U. Bhadra, S. K. Singh, S.N.C.V.L. Pushpavalli, P. B. Hajeri, and M. Pal-Bhadra

    RNA Interference and microRNAs in Zebra Fish, A. S. Flynt, E. J. Thatcher, and J. G. Patton

    Biogenesis and Function of Plant microRNAs, Z. Havelda

    Endogenous Small RNA Pathways in Arabidopsis, M. Agarwal, J. Curaba, and X. Chen

    How to Assay microRNA Expression: A Technology Guide, M. Castoldi, V. Benes, and M. U. Muckenthaler

    Methods to Quantify microRNA Gene Expression, L. A. Neely

    Regulation of Alternative Splicing by microRNAs, R. K. Gaur

    Recent Progress in Polymerase II-Mediated Intronic microRNA Expression Systems, S.-L. Lin and S.-Y. Ying

    MicroRNA-Based RNA Polymerase II Expression Vectors for RNA Interference in Mammalian Cells, A. B. Vojtek, K.-H. Chung, P. D. Patel, and D. L. Turner

    Transgenic RNAi: A Fast and Low-Cost Approach to Reverse Genetics in Mammals, L. Qiu and Z. Xu 

    Symphony of AIDS: A microRNA-Based Therapy, Y. R. Fujii

    MicroRNAs and Cancer: Connecting the Dots, S. D. Jayasena 

    Mammalian Transcriptional Gene Silencing by Small RNAs, D. H. Kim and J. J. Rossi

    Regulation of Gene Expression by RNA-Mediated Transcriptional Gene Silencing, K. V. Morris


    Rajesh K. Gaur, John J. Rossi