Revival: Biofilms (1995): Methods for Enzymatic Release of Microorganisms, 1st Edition (Paperback) book cover

Revival: Biofilms (1995)

Methods for Enzymatic Release of Microorganisms, 1st Edition

By Jean F. Brisou

CRC Press

218 pages

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This text presents a new technique for detecting microorganisms, specifically bacteria found in all levels of the biosphere. It also discusses methods for enzymatic release of these microbes as well as their interactions in all ecosystems. Drawings and micrographs help to illustrate this concept.

Part one is devoted to the mechanisms of adherence. The second part discusses microbial ecology and the bacterial population of tissues as well as both land and aquatic microbiocenoses in general. Part three specifically covers technique. What is known about the mechanisms of adherence justifies the choice of techniques suggested. Applications in areas such as nature, medicine, environmental hygiene and the food industry are discussed.

The explanation of useful techniques, the author's research results, and practical application methods make this volume an essential reference tool for researchers, technicians and practitioners.

Table of Contents

Part I: Adherence in Microbiology

Structures and Mechanisms


Review of Membrane Structures

Membrane Proteins

Renewal of Membrane Constituents

Adherence Mechanisms

Adsorption-Ionic Zone

The Roles of Polycations

Adhesion-Second Reversible Stage

Irreversible Adherence-Adhesins



Other Possible Adhesins


A Few Genetic Reminders

Chemical Elements of Adherence

The Bacterial Wall


Biosynthesis of Polysaccharides

Main Bacterial Polysaccharides




Polypeptides and Derivates

Lipoproteins and Derivative Complexes

Particularities of Mycobacteria

Composition Differences of the Bacterial Walls

Part II: Bacteria Life in the Wild

Adherence in Living Tissues

Cellular Receptors

Adherence to a Few Organs and Organisms

Man and Animals

Microbisms of the Mouth and Teeth

Microbisms of the Digestive Tube

Microbisms of the Stomach

Microbisms of the Urogenital Apparatus

Natural Microbiocenoses


The Rhizosphere

Soils and Sediments

Aquatic Environments

Gas/Liquid Interfaces

Nonmiscible Liquid Interfaces

Microbial Activity on Interfaces

Role of Plankton in Aquatic Environments

Polymorphism of Bacteria in Nature, Agglomerates

Inert Surfaces

Part III: Enzymatic Release Techniques of Bacteria

Basic Techniques


Attack of Host Sites or Substrates

Inorganic or Inert Surfaces

Organic Surfaces

Analysis of North Atlantic Seawater

Useable Enzymes

Optimal Conditions of Useable Enzymes

Practical Applications

Techniques and Methods

Quantitation of the Enzymes

Conservation of the Enzyme Solutions

Standard Method

Specific Method

Stages of Release

Experiments With Only Enzymes

On Marine Sediment

Experimentation on Mussel Grindings

Treatment of Infected Urine

Experiment on Inert Powders

Association of Enzymes-Antibiotics

Experiment Using Bacteria Marked With Radioelements

Special Case of Fat

Experiment by Richelle Maurer

Experiment by E. Ebiou

Experiment by Patrick Brisou




About the Author

Jean F. Brisou is a national corresponding member of the Academy of Medicine.

About the Series

CRC Press Revivals

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Biology / Microbiology
SCIENCE / Life Sciences / General