The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.
Table of Contents
Part I IMMUNOCYTOMAS in LOUVAIN (LOU) RATS.
The LOUVAIN (LOU) Rats.
Rat Immunocytomas (IR).
Part II PRODUCTION of RAT-RAT HYBRIDOMAS.
Fusion Cell Lines.
Immunization of Rats.
Rat Hybridoma Prodcution: Scanning electron Microscopy of a Fusion Experiment
Selective Growth of Hybrids.
Screening of Monoclonal Antibodies Directed Against Soluble Antigens
Screening of Hybrid Supernatants for Monoclonal Antibodies Against Cellular Antigens
Isotyping of Rat Monoclonal Antibodies.
Adaptation to In Vivo Culture. Freezing and Thawing.
Freezing and Thawing
Part III PRODUCTION, PURIFICATION, FRAGMENTATION and LABELING of MONOCLONAL ANTIBODIES.
In Vitro Production of Rat Monoclonal Antibodies.
In Vivo Production of Rat Monoclonal Antibodies.
Purification of Rat Monoclonal Antibodies from Ascitic Fluid - Serum or Culture Supernatant.
Quality Control of Purified Antibodies.
Fragmentation of Rat Monoclonal Antibodies.
Labeling of Monoclonal Antibodies.
Part IV EXAMPLES of PRODUCTION and CHARACTERIZATION of RAT-RAT HYBRIDOMAS AGAINST VARIOUS Ag.
Rat Monoclonal Antibodies Against Murine Immunoglobulins.
Allotype Specific Monoclonal Antibodies to Rat Kappa Light Chain.
Application of Rat Hybridomas to Studies on Ocular Immunobiology.
Rat Monoclonal Antibodies Against Rabbit Immunoglobulin.
Preparation of Anti-Human IgG Monoclonal Antibodies Using Rat x Rat Hybridoma System.
Rat Monoclonal Antibodies Against Human Immunoglobulin.
Production and Characterization of Rat Monoclonal Antibodies Directed Against Human Apolipoprotein B.
Studies on Rat-Rat Hybridoma Technique and its Application to Obtain Rat Monoclonal Antibodies Anti-Horse-radish Peroxidase.
Rat-Rat and Rat-Mouse Hybridomas Secreting Rat Monoclonal Anti-Horse-radish Peroxidase.
LOU Rat Monoclonal Antibodies Against Xenopus Vitellogenin.
Production and Characterization of Rat-Rat Hybridomas Against DNP-Hapten. Rat x Rat Hybridomas Against Cellular Antigens.
Rat Monoclonal Antibodies Against Human Leukocyte Differentiation Antigens.
Rat Monoclonal Antibodies Specific for T Lymphocytes.
Rat Monoclonal Antibodies Against Streptococcus mutans and Bacteroides gingivalis.
A Specific Rat Monoclonal Antibody Against Yersinia enterocolitica Serogroup 0:8.
Production of Rat Hybridoma Directed Against Yersinia enterocolitica.
Rat Monoclonal Antibodies Against Viral Antigens.
Contribution of Rat Monoclonal Antibodies to the Study of Schistosoma mansoni Infections.
Part V SPECIFIC USES and PROPERTIES of RAT MONOCLONAL ANTIBODIES.
Mouse Immunoglobulin Isotyping.
Humoral Immune Suppression of Mice by Monoclonal Rat Anti-Mouse µHeavy Chain Antibodies.
Rat Monoclonal Antibodies Against Mouse IgE.
Purification of Mouse Monoclonal Antibodies by Immunoaffinity Chromatography.
Screening of Mouse Monoclonal Antibodies.
Use of Rat Monoclonal Antibodies in Immunohistochemistry Investigations.
Use of Rat-Rat Monoclonal Antibodies Labeled with Fluorochrome.
Use of Rat Monoclonal Anti-Rat Allotype Antibodies in Immunohistoperoxidase Technique and ELISA.
Use of Anti-Rat Kappa Light Chain Allotype Monoclonal Antibodies for the Quantification of Rat Monoclonal Antibodies in Ascitic Fluid or Serum.
Extracorporeal Removal of Arteriogenic Particles by Use of Rat Monoclonal Antibody Coupled to Sepharose as Specific Immunosorbent.
Antibacteria Antigens, Especially Streptococcus mutans.
Mercury-Induced Autoimmunity Production of Monoclonal Autoantibodies.
Rat Monoclonal Antibodies to Plant Pathogens.
Rat Monoclonal Antibodies for Complement-Mediated and Cell-Medicated Cytolysis.
Rat Antibodies and K Cells.
Absence of Viral Particles in Rat Hybridomas - An Ultrastructural Study.
Role of CODATA-HDB in the Centralization and Distribution of Information Concerning the Rat Hybridomas.
List of Monoclonal Antibodies.
Herve Bazin is Professor Emeritus at Universite Catholique de Louvain.