1st Edition

Single-Molecule Tools for Bioanalysis

Edited By Shuo Huang Copyright 2022
    278 Pages 17 Color & 70 B/W Illustrations
    by Jenny Stanford Publishing

    278 Pages 17 Color & 70 B/W Illustrations
    by Jenny Stanford Publishing

    In the last three decades, the fast development of single-molecule techniques has revolutionized the way we observe and understand biological processes. Some of these techniques have been further adapted as tools for bioanalysis. This book summarizes and details the frontiers of the development of these tools as well as their applications. The contributors are young and established researchers in their respective fields. The main content originates from the lecture notes of a chemistry graduate course taught by the book editor at Nanjing University. This book is suitable to be used as a textbook for a high-level undergraduate or an entry-level graduate course. The systematically written content provides a thorough illustration of the mechanisms of each methodology presented.

    1. Single-Molecule Analysis by Biological Nanopores

    Yuqin Wang and Shuo Huang

    2. Optical Tweezers for Manipulation of Single Molecules

    Guangtao Song and Yan Zeng

    3. Single-Molecule Biosensing by Fluorescence Resonance Energy Transfer

    Ying Lu, Jianbing Ma, and Ming Li

    4. DNA Origami as Single-Molecule Biosensors

    Travis A. Meyer, Qinyi Lu, Kristin Weiss, and Yonggang Ke

    5. Single-Molecule Manipulation by Magnetic Tweezers

    Zilong Guo and Hu Chen

    6. Long-Time Recording of Single-Molecule Dynamics in Solution by Anti-Brownian Trapping

    Quan Wang, Elif Karasu, and Hugh Wilson


    Shuo Huang obtained his BS in physics from Nanjing University, China in 2006 and his PhD in biophysics from Arizona State University in 2011. From 2011 to 2015, he was a postdoctoral fellow at the University of Oxford. In 2015, Prof. Huang joined Nanjing University, where he is Professor at the School of Chemistry and Chemical Engineering, State Key Laboratory of Analytical Chemistry for Life Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC). His research focuses on the development of single-molecule sensing tools based on biological nanopores.