Spectral Techniques In Proteomics: 1st Edition (Hardback) book cover

Spectral Techniques In Proteomics

1st Edition

Edited by Daniel S. Sem

CRC Press

464 pages | 119 B/W Illus.

Purchasing Options:$ = USD
Hardback: 9781574445800
pub: 2007-03-30
eBook (VitalSource) : 9780429134289
pub: 2007-03-30
from $28.98

FREE Standard Shipping!


Facilitating the innovation, development, and application of new spectroscopic methods in proteomics, Spectral Techniques in Proteomics provides a broad overview of the spectroscopic toolbox that can be used, either with proteome or sub-proteome mixtures or with individual/purified proteins studied in parallel. It gives a modest overview of existing and proven techniques as well as a detailed examination of less established spectroscopic methods with studied speculation on future applications.

Intended for a broad audience of protein biochemists and biophysicists, the book adopts a wider definition of proteomics to include the systems-based study of proteomes and sub-proteomes involving proteins related through regulatory cascades, metabolic pathways, post-translational modifications, or associated biologic effect, as well as the parallel study of subsets such as proteins with associated protein folds (structural proteomics) or binding sites (chemical proteomics). Beginning by defining the scope of the field as is relevant to spectroscopists, the book then briefly reviews current commonly used spectroscopic methods. It covers separation techniques that typically precede ESI studies as well as MALDI MS/MS based protein identification. SELDI is also presented as a tool that combines separation techniques with MS analysis on the same chip. The book presents studies of protein-protein and protein-ligand interactions using NIR fluorescence, NMR, MS, and SPR. Recent developments in ICAT labeling strategies are addressed along with a discussion of metabolomics. A description of advances in structural proteomics using NMR, x-ray crystallography, and EPR precedes a final summary of current technology and future prospects of the science.

Analyzing the current state of the science and the future evolution of the field, Spectral Techniques in Proteomics applies a systems-based approach to studying the function and mechanism of proteins.


“Intended for protein biochemists and biophysicists, … describes spectroscopic tools for use with proteome or subproteome mixtures or with individual/purified proteins studied parallel. … It presents surface-enhanced laser desorption/ionization as a tool that combines separation techniques with MS analysis on the same chip. Studies of protein-ligand interactions using NIR fluorescence — In Biophotonics International, No. 81, June 2007

Table of Contents

The Scope of Proteomic and Chemical Proteomic Studies

The Systems-Based Approach to Proteomics and Chemical Proteomics, D.S. Sem

Similarities in Protein Binding Sites, H.O. Villar, M.R. Hansen, and R. Kho

Survey of Spectral Techniques Used to Study Proteins, D.S. Sem

Mass Spectral Studies of Proteome and Subproteome Mixtures

Capillary Electrophoresis—Mass Spectrometry for Characterization of Peptides and Proteins, C. Neusüß and M. Pelzing

Protein and Peptide Analysis by Matrix-Assisted Laser Desorption/Ionization Tandem Mass Spectrometry (MALDI MS/MS), E. Sachon and O. Nørregaard Jensen

Characterization of Glycosylated Proteins by Mass Spectrometry Using Microcolumns and Enzymatic Digestion, Per Hägglund and M.R. Larsen

Surface-Enhanced Laser Desorption/Ionization Protein Biochip Technology for Proteomics Research and Assay Development, S.R. Weinberger, L. Lomas, E. Fung, and C. Enderwick

An Approach to the Reproducibility of SELDI Profiling, W.S. Liggett, P.E. Barker, L.H. Cazares, and

O.J. Semmes

Protein–Protein (or Peptide) Interactions: Studies in Parallel and with Mixtures

Mass Spectrometric Applications in Immunoproteomics, A.W. Purcell, N.A. Williamson, A.I. Webb, and

K. Lau

Near-Infrared Fluorescence Detection of Antigen–Antibody Interactions on Microarrays, S. Vehary and Y. Garabet

Application of Shotgun Proteomics to Transcriptional Regulatory Pathways, A.L. Mosley and M.P. Washburn

Electrophoretic NMR of Protein Mixtures and its Proteomic Applications, Q. He, S.B. Thakur, and J. Spater

Chemical Proteomics: Studies of Protein–Ligand Interactions in Pools and Pathways

Characterizing Proteins and Proteomes Using Isotope-Coded Mass Spectrometry, U. Kota and M.B. Goshe

Surface Plasmon Resonance Biosensors’ Contributions to Proteome Mapping, R.L. Rich and D.G. Myszka

Application of In-Cell NMR Spectroscopy to Investigation of Protein Behavior and Ligand–Protein Interaction inside Living Cells, V. Dötsch

An Overview of Metabonomics Techniques and Applications, J.C. Lindon, E. Holmes, and J.K. Nicholson

Structural Proteomics: Parallel Studies of Proteins

NMR-Based Structural Proteomics, J.L. Markley

Leveraging X-Ray Structural Information in Gene Family-Based Drug Discovery: Application to Protein Kinases, M. Jacobs, H. Zuccola, B. Hare, A. Aronov, A. Pierce, and G. Bemis

EPR Spectroscopy in Genome-Wide Expression Studies, R.Cammack

Summary of Chapters and Future Prospects for Spectral Techniques in Proteomics, Summarized by D.S. Sem (from each contributor)

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
SCIENCE / Chemistry / Industrial & Technical
SCIENCE / Research & Methodology