1st Edition

Spin-Label Electron Paramagnetic Resonance Spectroscopy

By Derek Marsh Copyright 2020
516 Pages 318 B/W Illustrations
by CRC Press

514 Pages 318 B/W Illustrations
by CRC Press

472 Pages
by CRC Press

Spin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular probe method that finds wide application in molecular biophysics and structural biology. This book provides the first comprehensive summary of basic principles, spectroscopic properties, and use for studying biological membranes, protein folding, supramolecular structure, lipid-protein interactions, and... Read more

Chapter 1. Introduction

Chapter 2. The Nitroxide EPR Spectrum

Chapter 3. Hyperfine Interactions and G-Vaules

Chapter 4. Polarity Dependance

Chapter 5. Spin Relaxation Theory

Chapter 6. EPR Line-Shaped Theory

Chapter 7. Dynamics and Rotational Diffusion

Chapter 8. Dynamics and Orientational Ordering (Liquid Crystals and Membranes)

Chapter 9. Spin-Spin Interactions

Chapter 10. Spin-Latice Relaxation

Chapter 11. Nonlinear and Saturation-Transfr EPR

Chapter 12. Saturation-Recovery EPR and ELDOR

Chapter 13 Spin-Echo EPR

Chapter 14. ESEEM and ENDOR: Hyperfine Spectroscopy

Chapter 15. Distance Measurements

Chapter 16. Site-Directed Spin Labelling (SDSL)

Biography

Derek Marsh has published hundreds of original papers in this area, over its 40-year period of development, and has also contributed several reviews and book chapters in this area. He is coauthor of the (now out-of-print) student-level textbook Magnetic Resonance of Biomolecules (1976) and author of the reference work Handbook of Lipid Bilayers (Second Edition to be published in spring 2012).

His studies were performed at the University of Oxford, and he earned a B.A. and M.A. in Physics, and a D.Phil. in Magnetic Resonance (1971). His graduate research focused on magnetic resonance (ESR) spectroscopy applied to magnetic investigations of transition metals in solids. He was a postdoctoral researcher in the Astbury Department of Biophysics at the University of Leeds, using magnetic resonance to study the structure and function of phospholipid bilayers and biological membranes. He also had appointments at the National Research Council of Canada, Max-Planck Institute for Biophysical Chemistry, and the Department of Biochemistry at the University of Oxford. He has been on the permanent research staff of the Max-Planck Institute for Biophysical Chemistry since 1975, and was Visiting Professor at the University of Leeds from 1997 to 2010.

Dr. Marsh has been on the editorial board of numerous journals, including Chemistry and Physics of Lipids, Biochimica et Biophysica Acta, Biochemical Journal, and Biophysical Journal. He has authored or co-authored over 425 scientific papers to date.