While the genomic revolution has quickly led to the deposit of more than 30,000 structures in the protein data bank (PDB), less than one percent of those contributions represent membrane proteins despite the fact that membrane proteins constitute some 20 percent of all proteins. This discrepancy becomes significantly troublesome when it is coupled with the fact that 60 percent of current drugs are based on targeting this group of proteins, a trend that does not seem likely to reverse.
Structural Genomics on Membrane Proteins provides an excellent overview on novel research in bioinformatics and modeling on membranes, as well as the latest technological developments being employed in expression, purification, and crystallography to obtain high-resolution structures on membrane proteins. This cutting-edge work also explains the difficulties facing researchers—both technical and ethical—that have slowed the process.
Structural Genomics on Membrane Proteins provides researchers with an unprecedented look at the novel technologies that will ultimately allow them to conquer the last frontier in structural biology, leading to accelerated breakthroughs in drug discovery.
Table of Contents
Introduction. Bioinformatics in Membrane Protein Analysis. Prokaryotic Membrane Transport Proteins: Amplified Expression and Purification. Membrane Protein Production Strategies for Structural Genomics. Refolding of Membrane Proteins for Large-scale Production. Crystallization of Membrane Proteins. Signaling through Membrane Proteins. Expression of Membrane Proteins in Yeasts. Expression of Functional Membrane Proteins in the Baculovirus-Insect Cell System: Challenges and Developments. Expression of Membrane Proteins in Mammalian Cells, Solubilization and Purification of Membrane Proteins. Fluorescent Labeling of Membrane Proteins in Living Cells. Membrane Protein NMR. Miniaturization of Structural Biology Technologies: From Expression to Biophysical Analyses. Electron and Atomic Force Microscopy of Reconstituted Membrane Proteins. Structural Genomics on Membrane Proteins. Molecular Modeling of Membrane Proteins. Towards Structural Bases for GPCR Ligand-binding: A Path for Drug Discovery.
Kenneth H. Lundstrom