Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book—Techniques in Genetic Engineering—IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and genomes; however, in general it is assumed that the reader has a background on these key issues.
Techniques in Genetic Engineering briefly introduces some common genetic engineering techniques and focuses on how to approach different real-life problems using a combination of these key issues. Although not an exhaustive review of these techniques, basic information includes core concepts such as DNA, RNA, protein, genes, and genomes. It is assumed that the reader has background on these key issues. The book provides sufficient background and future perspectives for the readers to develop their own experimental strategies and innovations.
This easy-to-follow book presents not only the theoretical background of molecular techniques, but also provides case study examples, with some sample solutions. The book covers basic molecular cloning procedures; genetic modification of cells, including stem cells; as well as multicellular organisms, using problem-based case study examples.
Table of Contents
Introduction to Genetic Engineering. Tools of Genetic Engineering. DNA libraries. Protein production and purification. Mutagenesis. Protein-protein interactions. Cell Culture. Genetic Manipulation of Animals. Genetic Manipulation of Plants. Today and the Future. Appendix I – DNA techniques. Appendix II – RNA techniques. Appendix III – Protein Techniques.
Prof. Işıl AKSAN KURNAZ has received her BSc from Bogazici University, Department of Molecular Biology and Genetics, after which she did her PhD with Dr. Colin Goding in Marie Curie Research Institute, UK (closed down in 2010) and University of London, Institute of Cancer Research. Following her degree, she has worked as a lecturer in Bogazici University (1999-2000), and a postdoctoral researcher with Prof. Andrew D. Sharrocks in University of Manchester (2000-2002). She was one of the two young assistant professors to be then recruited by Yeditepe University in Istanbul, Turkey, so as to set up biotechnology and genetics laboratories as well as the Department of Genetics and Bioengineering in 2003. She continued to work there as a professor and principle investigator of the Molecular Neurobiology Laboratory (aka AxanLab, https://www.facebook.com/AxanLab) until 2014, when she relocated her laboratory to Gebze Institute of Technology (GYTE) in Kocaeli, Turkey.
Prof. Kurnaz is the recipient of the L’Oreal Turkey Young Female Investigator Award (as a local counterpart of the international For Women in Science programme) in 2006, and the GEBIP Award (Genc Bilim Insanlarini Destekleme Programi / Distinguished Young Investigator Award) of the Turkish Academy of Sciences (TUBA) in 2007. She has also participated in the European Young Academy brainstorming meeting organized by ALLEA and ESF in Vienna, 2009. She has been involved in several international grants such as COST and FP7 Regpot projects, as well as many national grants from TUBITAK, Turkish Fight with Cancer Foundation, Brain Research Society – Lundbeck, Novartis among many others. She has published numerous papers, to which she has received over 500 citations, with a current h index of 9.