6th Edition

Textbook of Assisted Reproductive Techniques Volume 1: Laboratory Perspectives

    362 Pages 121 Color & 60 B/W Illustrations
    by CRC Press

    362 Pages 121 Color & 60 B/W Illustrations
    by CRC Press

    Established as the definitive reference for the IVF clinic, the sixth edition has been extensively revised, with the addition of several important new contributions on laboratory topics, including KPIs for the IVF laboratory, Quality control in the cloud, Artificial Intelligence, AI in gamete and embryo selection, Demystifying vitrification, Microfluidics, Gene editing, Disaster management, and Early human embryo development revealed by static imaging. As previously, methods, protocols, and techniques of choice are presented by IVF pioneers and eminent international experts.


    List of contributors

    1. Updated Guidelines for Setting up an Assisted Reproductive Technology Laboratory - Jacques Cohen, Mina Alikani, and Antonia Gilligan

    2. Quality Control: Maintaining Stability in the Laboratory - Ronny Janssens, Neelke De Munck, and Johan Guns

    3. KPIs for the IVF Laboratory - Alison Campbell

    4. QC in the Clouds—Digitizing Quality Control - Giles Anthony Palmer

    5. The Assisted Reproduction Technology Laboratory: Current Standards - Cecilia Sjöblom

    6. Evaluation of Sperm - Chelsey A. Leisinger, Kaylen M. Silverberg, and Matthew D. VerMilyea

    7. Sperm Preparation Techniques and Advanced Sperm Selection for Intracytoplasmic Sperm Injection - Sinan Ozkavukcu, George Hughes, and Christopher LR Barratt

    8. Sperm Chromatin Assessment - Ashok Agarwal and Rakesh Sharma

    9. Oocyte Retrieval and Selection - Laura Rienzi, Rosaria Gentile, Federica Innocenti, Danilo Cimadomo, Alberto Vaiarelli, and Filippo Maria Ubaldi

    10. Preparation and Evaluation of Oocytes for Intracytoplasmic Sperm Injection - Irit Granot and Nava Dekel

    11. Use of IVM in a Clinical Setting: What is its Role in ART? - Tuong M Ho and Lan N Vuong

    12. Intracytoplasmic Sperm Injection: Technical Aspects - Sydney Souness, Philip Xie, Stephanie Cheung, Olena M. Kocur, Lily Ng, Zev Rosenwaks, and Gianpiero D. Palermo

    13. Human Embryo Biopsy Procedures - Maurizio Poli, Ludovica Picchetta, and Antonio Capalbo

    14. Analysis of Fertilization - Thomas Ebner

    15. Culture Systems for the Human Embryo -  David K. Gardner

    16. Embryo Selection through the Analysis of Morphology and Physiology - Denny Sakkas and David K. Gardner

    17. Evaluation of Embryo Quality: Time-Lapse Imaging to Assess Embryo Morphokinesis - Akhil Garg, María Ángeles Valera, and Marcos Meseguer

    18. Artificial Intelligence (AI) - Daniella Gilboa

    19. Artificial Intelligence (AI) in Gamete and Embryo Selection - Chloe He, Neringa Karpaviciute, Cristina Hickman, and Nikica Zaninovic

    20. Demystifying Vitrification - Debra A. Gook and Kelly Lewis

    21. Vitrification of the Human Oocyte - Masashige Kuwayama

    22. The Human Embryo: Vitrification - Zsolt Peter Nagy and Ching-Chien Chang

    23. Managing an Oocyte Bank - Ana Cobo, Pilar Alamá, José María De Los Santos, María José De Los Santos, and José Remohí

    24. Severe Male Factor Infertility: Genetic Consequences and Recommendations for Genetic Testing - Katrien Stouffs

    25. Pre-Implantation Genetic Testing for Aneuploidy to Improve Clinical Outcomes - Jenna S. Hynes, Eric J. Forman, and Alan H. Handyside

    26. Diagnosis of Endometrial Receptivity, Embryo–Endometrial Dialogue, and Endometrial Microbiome - Maria Ruiz-Alonso, Inmaculada Moreno, Felipe Vilella, and Carlos Simon

    27. Artificial Gametes: Oocytes - Evelyn E Telfer and Yvonne L Odey

    28. How Microfluidics and Microfabrication will Improve Diagnosis and Treatment in Human Art - Dale M. Goss, Steven A. Vasilescu, Majid E. Warkiani, Jeremy G. Thompson, and David K. Gardner

    29. Genome Editing in Human Reproduction - Helen C O’Neill

    30. Designing Disaster Plans for IVF Laboratories - Kimball O Pomeroy and Michael L Reed

    31. Early Human Embryo Development Revealed by Static Imaging - Yanina D. Alvarez and Melanie D. White



    David K. Gardner AM, FAA, DPhil is a Professor in the School of BioSciences at the University of Melbourne, and is the Scientific Director of Melbourne IVF, Melbourne, Australia.

    Ariel Weissman MD is a Senior Physician at the IVF Unit, Department of Obstetrics and Gynecology, Edith Wolfson Medical Center, Holon, and a full Professor of Obstetrics and Gynecology at the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

    Colin M. Howles PhD, FRSM is based at Aries Consulting SARL, Geneva, Switzerland, and is an Honorary Fellow, University of Edinburgh, UK.

    Zeev Shoham MD is a Professor at the Hebrew University, Jerusalem, Israel, a consultant in IVF, and co-founder of IVF-Worldwide.