The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology, 1st Edition (Hardback) book cover

The Dynamic Synapse

Molecular Methods in Ionotropic Receptor Biology, 1st Edition

Edited by Josef T. Kittler, Stephen J. Moss

CRC Press

336 pages | 96 B/W Illus.

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pub: 2006-03-27
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Exploring the diverse tools and technologies used to study synaptic processes, The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology delineates techniques, methods, and conceptual advances for studying neurotransmitter receptors and other synaptic proteins. It describes a broad range of molecular, biochemical, imaging, and electrophysiological approaches for studying the biology of synapses.

Specific topics include the use of proteomics to study synaptic protein complexes, the development of phosphorylation state specific antibodies, post-genomic tools applied to the study of synapses and RNA interference in neurons. In addition, several chapters focus on methods for gene and protein delivery into neuronal tissue. The use of biochemical, electrophysiological and optical tagging techniques to study the movement and membrane trafficking of neurotransmitter receptors in the membrane of live nerve cells are also discussed. To complement these approaches, the application of approaches for achieving long-term alterations in the genetic complement of neurons in vivo using viral vectors or homologous recombination of ES cells are also described.

Table of Contents

Methods for Uncovering the Mechanisms of AMPA Receptor Trafficking, S. Restituito and E.B. Ziff

Long-Term Plasticity at Inhibitory Synapses: A Phenomenon That Has Been Overlooked, J.-L. Gaiarsa and Y. Ben-Ari

New Tricks for an Old Dog: Proteomics of the PSD, B.A. Jordan, B.D. Fernholz, T.A. Neubert, and E.B. Ziff

Phosphorylation Site-Specific Antibodies as Research Tools in Studies of Native GABAA Receptors, J.N. Jovanovic

Protein Palmitoylation by DHHC Protein Family, Y. Fukata, D.S. Bredt, and M. Fukata

Studying the Localization, Surface Stability and Endocytosis of Neurotransmitter Receptors by Antibody Labeling and Biotinylation Approaches, I.L. Arancibia-Cárcamo, B.P. Fairfax, S.J. Moss, and J.T. Kittler

Visualization of AMPAR Trafficking and Surface Expression, P.V. Perestenko and J.M. Henley

Neurotransmitter Dynamics, S. Lévi and A. Triller

Receptor Dynamics at the Cell Surface Studied Using Functional Tagging, P. Thomas and T.G. Smart

RNAi and Applications in Neurobiology, T.C. Jacob

Transfecting and Transducing Neurons with Synthetic Nucleic Acids and Biologically Active Macromolecules, J.T. Kittler, J.G. Hanley, and J.T.R. Isaac

Acute In Vivo Expression of Recombinant Proteins in Rat Brain Using Sindbis Virus, H. Marie and R.C. Malenka

Lentivirus-Based Genetic Manipulations in Neurons In Vivo, P. Osten, T. Dittgen, and P. Licznerski

AMPA Receptor Phosphorylation in Synaptic Plasticity: Insights from Knockin Mice, H.-K. Lee

Genomic and Post-Genomic Tools for Studying Synapse Biology, J.T. Kittler and P.L. Oliver


About the Series

Frontiers in Neuroscience

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Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Anatomy
SCIENCE / Life Sciences / Cytology
SCIENCE / Life Sciences / General
SCIENCE / Life Sciences / Neuroscience