The Giant Vesicle Book: 1st Edition (Hardback) book cover

The Giant Vesicle Book

1st Edition

Edited by Rumiana Dimova, Carlos Marques

CRC Press

634 pages | 67 Color Illus. | 172 B/W Illus.

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Hardback: 9781498752176
pub: 2019-09-05
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Giant vesicles are being widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials, in particular for drug delivery, as well as use in driving new technologies for the cosmetics industry. This practical manual gives the reader a toolbox for starting work with giant vesicles, including expert’s tips on proper preparation methods, measurement, and characterization methods. The contents build from a simple model to use of vesicles as advanced membrane and cell system models. It also includes fundamentals for understanding vesicle structure, properties and behavior.

Key Features

• Provides a complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications.

• Emphasizes clarity of presentation with instructive illustrations and exemplary data.

• Covers different preparation methods for simple to more complex membrane models.

• Discusses the main techniques for characterization and measurement of membrane properties.

• Includes handy tables with data and structures for ready reference.

Table of Contents

Preparation methods for giant unilamellar vesicles. Preparation and properties of giant plasma membrane vesicles and giant unilamellar vesicles from natural membranes. Protein reconstitution in giant vesicles. GUVs with cytoskeleton. Understanding giant vesicles – a theoretical perspective. Simulating membranes, vesicles, and cells. Theory of vesicle dynamics in flow and electric fields. Particle-membrane interactions. Theory of polymer-membrane interactions. Application of optical microscopy techniques on giant unilamellar vesicles. Mechanics assays of synthetic lipid membranes based on micropipette aspiration. Atomic force microscopy of giant unilamellar vesicles. Manipulation and biophysical characterization of GUVs with an optical stretcher. Vesicle fluctuation analysis. Using electric fields to assess membrane material properties in GUVs. Creating membrane nanotubes from GUVs. Measuring GUV adhesion. Phase diagrams and tie lines in GUVs. Vesicle dynamics in flow: an experimental approach. Membrane permeability measurements. Lipid and protein mobility in GUVs. Shining light on membranes. Protein-membrane interactions. Effects of antimicrobial peptides and detergents on guvs. Lipid-polymer interactions: effect on GUVs shapes and behavior. Polymersomes. Giant hybrid polymer/lipid vesicles. Giant unilamellar vesicles: from protocell models to the construction of minimal cells. Encapsulation of aqueous two-phase systems and gels within giant lipid vesicles. Droplet-supported giant lipid vesicles as compartments for synthetic biology. List of lipids and physical constants of lipid bilayers. List of membrane dyes and fluorescent groups conjugated to lipids. List of detergents. List of water-soluble dyes or their fluorescent groups and their structures

About the Editors

Rumiana Dimova leads an experimental lab in biophysics at the Max Planck Institute of Colloids and Interfaces in Potsdam, Germany. She has been working with giant vesicles already during her MSc studies and was introduced to the magic of their preparation by M. Angelova, who established the well-known electroformation protocol for giant vesicle formation. Rumiana’s PhD aimed at developing biophysical approaches to assess membrane rheological properties. Since then, she has been pursuing a variety of projects employing giant vesicles as a platform to develop new methods for the biophysical characterization of membranes and processes involving them. These studies have resulted in more than hundred peer-reviewed publications and book contributions. In 2017, she was nominated as the chair of the Membrane Structure and Assembly Subgroup of the Biophysical Society. Recently, she was also awarded the Emmy Noether distinction for women in physics of the European Physical Society.

Carlos Marques, a CNRS senior scientist, founded the MCube group at the Charles Sadron Institute in Strasbourg, France, where he gears experimental and theoretical research towards the understanding of the physical properties of self-assembled lipid bilayers. Trained as a polymer theoretician, Carlos first got interested in membranes because they interact with polymers and published the first prediction for the membrane changes expected when polymers adsorb on lipid bilayers. He then expanded the scope of his group to include experiments and numerical simulations, and has now published over one hundred thirty scientific papers, dealing with a variety of facets of Soft Condensed Matter, and in particular with lipid bilayers. He published many papers based on research with giant unilamellar vesicles, including the first study of lipid oxidation in GUVs and the discovery of the so-called PVA method for vesicle growth.

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
SCIENCE / Research & Methodology
SCIENCE / Physics