1st Edition
The Metabolic Pathway Engineering Handbook Tools and Applications
Volume II
Section VI. Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-Dannert
Evolutionary
engineering of industrially important microbial phenotypes
Expanding
enzyme function and activity through molecular evolution
Evolving
the function of DNA and RNA regulatory regions through molecular evolution
Evolving
pathways and genomes for the production of natural and novel compounds
Models
predicting optimized evolutionary strategies
Section VII. Gene Expression Tools for Metabolic Pathway Engineering,
Low-copy
number plasmids as artificial chromosomes
Chromosomal
engineering strategies
Regulating
gene expression through engineered RNA technologies
Tools
designed to regulate translational efficiency
Regulating
gene expression through post-translational modifications
Engineering
bifunctional enzymes for optimized metabolite transfer between sequential
conversion steps
Practical
pathway engineering – demonstration in integrating tools
Section VIII. Applications of Emerging Technologies to Metabolic
Engineering, Jay Keasling
Genome-wide
technologies: DNA and phenotypic microarrays, proteomics
Monitoring
and measuring the metabolome
Section IX. Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup Lee
Genome-scale
models, systems biology, and metabolic engineering
Cell-free
systems for metabolic engineering
Dynamic in
silico cell models
Appendix I: Tools for Experimentally Determining Flux through Pathways,
Measuring
flux by isotope labeling
Tools for
measuring intermediate and product formation
Quantitative analysis of labeling data
Appendix II: Applications, Brian Pfleger and Brian Brazeau
Cofactor regulation in
biocatalytic redox reactions
Metabolic engineering for fine
chemical production
Metabolic engineering for drug
production
Metabolic engineering for
alternative fuels
Biography
Christina Smolke
