The Metabolic Pathway Engineering Handbook : Tools and Applications book cover
1st Edition

The Metabolic Pathway Engineering Handbook
Tools and Applications

Edited By

Christina Smolke

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ISBN 9781138114715
Published August 30, 2017 by CRC Press
582 Pages 15 Color & 124 B/W Illustrations

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Book Description

This second volume of the Metabolic Pathway Engineering Handbook delves into evolutionary tools and gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. This volume also looks at emerging applications for producing fine chemicals, drugs, and alternative fuels.

Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.

Table of Contents

Volume II

Section VI. Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-Dannert

Evolutionary engineering of industrially important microbial phenotypes

Expanding enzyme function and activity through molecular evolution

Evolving the function of DNA and RNA regulatory regions through molecular evolution

Evolving pathways and genomes for the production of natural and novel compounds

Models predicting optimized evolutionary strategies

Section VII. Gene Expression Tools for Metabolic Pathway Engineering, Christina Smolke

Low-copy number plasmids as artificial chromosomes

Chromosomal engineering strategies

Regulating gene expression through engineered RNA technologies

Tools designed to regulate translational efficiency

Regulating gene expression through post-translational modifications

Engineering bifunctional enzymes for optimized metabolite transfer between sequential conversion steps

Practical pathway engineering – demonstration in integrating tools

Section VIII. Applications of Emerging Technologies to Metabolic Engineering, Jay Keasling

Genome-wide technologies: DNA and phenotypic microarrays, proteomics

Monitoring and measuring the metabolome

Section IX. Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup Lee

Genome-scale models, systems biology, and metabolic engineering

Cell-free systems for metabolic engineering

Dynamic in silico cell models

Appendix I: Tools for Experimentally Determining Flux through Pathways, Ralf Takors

Measuring flux by isotope labeling

Tools for measuring intermediate and product formation

Quantitative analysis of labeling data

Appendix II: Applications, Brian Pfleger and Brian Brazeau

Cofactor regulation in biocatalytic redox reactions

Metabolic engineering for fine chemical production

Metabolic engineering for drug production

Metabolic engineering for alternative fuels

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