The fourth edition of this work emphasizes the general practices and instrumentation involving TLC and HPTLC, as well as their applications based on compound types, while providing an understanding of the underlying theory necessary for optimizing these techniques. The book details up-to-date qualitative and quantitative densitometric experiments on organic dyes, lipids, antibiotics, pharmaceuticals, organic acids, insecticides, and more.
PART I: GENERAL PRACTICES OF TLC, 1. Introduction and History 2. Mechanism and Theory 3. Sorbents, Layers, and Precoated Plates 4. Obtaining Material for TLC and Sample Preparation 5. Application of Samples 6. Solvent Systems 7. Development Techniques 8. Detection and Visualization 9. Qualitative Evaluation and Documentation 10. Quantification 11. Reproducibility and Validation of Results 12. Preparative Layer Chromatography 13. Radiochemical Techniques PART II: APPLICATIONS OF TLC TO DIFFERENT COMPOUND TYPES, 14. Basic TLC Design and TLC of Organic Dyes 15. Lipids 16. Amino Acids 17. Carbohydrates 18. Natural Pigments 19. Vitamins 20. Nucleic Acid Derivatives 21. Steroids and Terpinoids 22. Pharmaceuticals 23. Miscellaneous Applications