Thin Layer Chromatography in Drug Analysis  book cover
1st Edition

Thin Layer Chromatography in Drug Analysis

ISBN 9781466507159
Published December 20, 2013 by CRC Press
1067 Pages - 351 B/W Illustrations

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Book Description

Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation.

The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market—the presence of substandard and counterfeit pharmaceutical products.

Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.

Table of Contents

Part I Theory of Thin Layer Chromatography in Context of Pharmaceutical Analysis

Overview of Drug Analysis and Structure of the Book
Łukasz Komsta, Monika Waksmundzka-Hajnos, and Joseph Sherma

Chemistry of Drugs and Its Influence on Retention
Oliwia Szerkus and Michał J. Markuszewski

Sorbents and Layers Used in Drug Analysis
Fred Rabel and Joseph Sherma

Optimization of Mobile Phase Composition
Mirosław Hawrył and Anna Hawrył
Chromatographic Analysis of Ionic Drugs
Monika Waksmundzka-Hajnos

Ionic Liquid Additives to Mobile Phases
Roman Kaliszan and Michał Piotr Marszałł
Chromatographic Analysis of Chiral Drugs
Shuchi Dixit and Ravi Bhushan

Chambers, Sample Application, and New Devices in the Chromatography of Drugs
Beata Polak, Radosław Gwarda, Ewelina Kopciał, and Tadeusz H. Dzido
2D Planar Chromatography
Huba Kalász

Quantitative Detection of Drugs by Densitometry and Video Scanning
Bernd Spangenberg

Statistical Evaluation and Validation of Quantitative Methods of Drug Analysis
Łukasz Komsta

Detection and Identification in TLC Drug Analysis
Łukasz Cieśla and Iwona Kowalska

TLC Determination of Drug Lipophilicity
Živoslav Lj. Tešic and Dušanka M. Milojkovic-Opsenica

Screening of Substandard and Fake Drugs in Underdeveloped Countries by TLC
Eliangiringa Kaale, Peter Risha, Thomas Layloff, and Joseph Sherma

Part II Planar Chromatography of Particular Drug Groups

TLC of Antidepressants and Neuroleptics
Robert Skibiński

TLC of Anxiolytics and Sedatives
Claudia Cimpoiu and Anamaria Hosu

TLC of Morphine Analogs
Grzegorz Jóźwiak

TLC of Nonopioid Analgesics, Anti-Inflammatics, and Antimigraine Drugs
Arkadiusz Pomykalski

TLC of Ergot Alkaloid Derivatives
Anna Petruczynik

Thin-Layer Chromatography of Anesthetics
Maja M. Natić
TLC of Psychostimulants
Joanna Drozd

TLC of Antiepileptics
Beata Paw

TLC of Alzheimer’s Disease Medicines
Łukasz Ciesla

TLC of Antiparkinsonians
Sigrid Mennickent, Marta de Diego, Mario Vega, and Carmen Gloria Godoy

Thin-Layer Chromatography of Cardiac Drugs
Rafał Pietraś
TLC of Antihypertensive and Antihypotensive Drugs
Danica Agbaba and Katarina Nikolić
TLC of Beta-Blockers and Beta-Agonists
Anna Gumieniczek and Anna Berecka

TLC of Antithrombotics
Marica Medić-Šarić, Mirza Bojić, and Željko Debeljak

TLC of Antihyperlipidemics
Łukasz Komsta

TLC of Spasmolytics
Przemysław Talik and Jan Krzek

TLC of Mucolytic, Antitussive, and Antiasthmatic Drugs
Radosław J. Ekiert and Jan Krzek

TLC of Neuromuscular Blockers
Dušanka M. Milojković-Opsenica and Maja M. Natić
TLC of Antiulcers
Tadeusz Inglot

TLC of Antiemetic Drugs
Łukasz Komsta

TLC of Steroids and Analogs
Rada M. Baošić
TLC of Drugs Used in Obesity and Sexual Dysfunction Treatment
Łukasz Komsta

TLC of Prostaglandins
Mariusz Stolarczyk, Anna Apola, and Jan Krzek

TLC of Diuretics
Urszula Hubicka, Anna Maslanka, and Jan Krzek

TLC of Antidiabetics
Anna Gumieniczek and Anna Berecka

TLC of Antihistamines
Danica Agbaba and Gordana Popović
TLC of Vitamins Including Nicotinic Acid Derivatives
Alina Pyka

TLC of Antiseptics
Anna Apola, Mariusz Stolarczyk, and Jan Krzek

TLC of Sulfonamides
Irena Choma and Wioleta Jesionek

TLC of Quinolones
Dorota Kowalczuk

Thin-Layer Chromatography of Tuberculostatic Drugs
Anamaria Hosu and Claudia Cimpoiu

TLC of Antifungal and Antiprotozoal Drugs
Živoslav Lj. Tešić and Jelena Đ. Trifković
TLC of β-Lactam Antibiotics
Monika Dąbrowska and Małgorzata Starek

TLC of Other Antibiotics
Małgorzata Starek and Monika Dąbrowska

Thin-Layer Chromatography of Antiviral Drugs
Anna Gumieniczek and Anna Berecka

Thin-Layer Chromatography of Anticancer Drugs
Duygu Yeniceli

Uncertainty Factors in the Enantioseparation of Chiral Drugs on Silica Gel Layers
Mieczysław Sajewicz and Teresa Kowalska

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Łukasz Komsta currently serves as assistant professor in the Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Poland. His research interests include the theory and application of liquid chromatography, especially in connection with computational chemistry and chemometrics, as well as the application of chromatographic methods, especially thin layer chromatography (TLC), in the analysis of drugs. Dr. Komsta is the author or coauthor of more than 80 scientific papers and about 70 scientific conference papers. Since 2011, he has been a member of the editorial board of the journal Acta Chromatographica. He also serves as section editor for the Polish scientific journal Current Issues in Pharmacy and Medical Sciences.

Monika Waksmundzka-Hajnos currently serves as full professor of pharmacy and head of the Department of Inorganic Chemistry at the Faculty of Pharmacy of the Medical University of Lublin, Poland. Her research interests include the theory and application of liquid chromatography, taking into consideration the optimization of chromatographic systems for the separation and quantitation of drugs and their degradation products in pharmaceutical preparations and drugs and their metabolites in body fluids, as well as isolation and/or separation of secondary metabolites in extracts of plant tissues. Professor Waksmundzka-Hajnos is the author or coauthor of about 150 papers, approximately 300 conference papers, and several chapters in known chromatographic textbooks. She is editor of the journal Acta Chromatographica and a member of the editorial board of Journal Planar Chromatography – Modern TLC. Since 2011, she has been a member of the editorial board of The Scientific World Journal—Analytical Chemistry. Professor Waksmundzka-Hajnos is the co-editor of two books from the Chromatographic Science Series: Thin-Layer Chromatography in Phytochemistry (2008, with Teresa Kowalska and Joseph Sherma) and High Performance Liquid Chromatography in Phytochemical Analysis (2010, with Joseph Sherma).

Joseph Sherma is currently John D. and Francis H. Larkin Professor Emeritus of Chemistry at Lafayette College, Easton, Pennsylvania. Professor Sherma has authored, coauthored, edited, or coedited more than 750 publications, including research papers and review articles in peer- reviewed journals; approximately 30 invited book chapters; and more than 70 books and U.S. government agency manuals in the areas of analytical chemistry and chromatography. In addition to his research in the techniques and applications of thin layer chromatography, Professor Sherma has a very productive interdisciplinary research program in the use of analytical chemistry to study biological systems with Biology Professor Emeritus Bernard Fried. Professor Sherma served for 23 years as editor for residues and trace elements of the Journal of AOAC International and is currently that journal’s Acquisitions Editor.