Thin Layer Chromatography in Phytochemistry: 1st Edition (Hardback) book cover

Thin Layer Chromatography in Phytochemistry

1st Edition

Edited by Monika Waksmundzka-Hajnos, Joseph Sherma, Teresa Kowalska

CRC Press

896 pages | 265 B/W Illus.

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pub: 2008-03-04
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Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants.

Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology.

Elucidates the role of plant materials in the pharmaceutical industry…

Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection.

Demonstrates practical methods that can be applied to a wide range of disciplines…

From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Table of Contents


Overview of the Field of TLC in Phytochemistry and the Structure of the Book; M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska

Plant Materials in Modern Pharmacy and Methods of Their Investigations; K. Skalicka-Wozniak, J. Widelski, and K. Głowniak

Medicines and Dietary Supplements Produced from Plants; A. Ankli, V. Widmer, and E. Reich

Primary and Secondary Metabolites and Their Biological Activity; I. Chinou

Plant Chemosystematics; C. Zidorn

Sorbents and Precoated Layers for the Analysis and Isolation of Primary and Secondary Metabolites; J. Sherma

Chambers, Sample Application, and Chromatogram Development; T.H. Dzido and T. Tuzimski

Derivatization, Detection (Quantification), and Identification of Compounds Online; B. Spangenberg

Biodetection and Determination of Biological Activity of Natural Compounds; E. Tyihák, Á. M. Móricz, and P.G. Ott

Forced-Flow Planar Layer Liquid Chromatographic Techniques for the Separation and Isolation of Natural Substances; E. Mincsovics


Primary Metabolites

TLC of Carbohydrates; G.L. Sassaki, L.M. de Souza, Thales R. Cipriani, and M. Iacomini

TLC of Lipids; S. Momchilova and B. Nikolova-Damyanova

Amino Acids; R. Bhushan

Secondary Metabolites—Shickimic Acid Derivatives

Sample Preparation and TLC Analysis of Phenolic Acids; M. Wójciak-Kosior and A. Oniszczuk

Application of TLC in the Isolation and Analysis of Coumarins; M. Waksmundzka-Hajnos and M.A. Hawrył

Application of TLC in the Isolation and Analysis of Flavonoids; M. Medić-Šarić, I. Jasprica, A. Mornar, and Z. Maleš

TLC of Lignans; L. Opletal and H. Sovová

Secondary Metabolites—Isoprenoids

TLC of Mono- and Sesquiterpenes; A. Koch, S. Basar, and R. Richter

TLC of Diterpenes; M. Hajnos

TLC of Triterpenes (Including Saponins); W. Oleszek, I. Kapusta, and A. Stochmal

TLC of Carotenoids; G. Britton

TLC of Sterols, Steroids, and Related Triterpenoids; L. Dinan, J. Harmatha, and R. Lafont

TLC of Iridoids; G. Zgórka

Secondary Metabolites—Amino Acid Derivatives

TLC of Indole Alkaloids; P.J. Houghton

TLC of Isoquinoline Alkaloids; M. Waksmundzka-Hajnos and A. Petruczynik

TLC of Tropane Alkaloids; T. Mroczek

TLC of Alkaloids from the Other Biosynthetic Groups; J. Flieger

Secondary Metabolites—Compounds Derived from Acetogenine (Acetylocoenzyme A)

Polyacetylenes: Distribution in Higher Plants, Pharmacological Effects, and Analysis; Lars P. Christensen and H.B. Jakobsen

Quinone Derivatives in Plant Extracts; G. Matysik, A. Skalska-Kaminska, and A. Matysik-Wozniak


About the Series

Chromatographic Science Series

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Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Botany
SCIENCE / Chemistry / General
SCIENCE / Chemistry / Industrial & Technical