This comprehensive publication draws together the important aspects of carcinogen-cell interaction. This interesting work describes the regulation of normal cell proliferation, cellular responses to carcinogen damage, the biologic consequences of cellular DNA modification, the structural and genomic changes resulting from carcinogen insult, and the possible involvement of such changes in the conversion of normal human fibroblasts to malignant cells. It presents current reviews with up-to-date literature references and provides critical information from leading investigators that is essential in understanding the biology of human cell transformation. This volume is especially useful to advanced students in genetic toxicology, molecular biologists, and all who are interested in the molecular and macromolecular changes in human cells leading to neoplasia.
Preface. Variations in Susceptibility to Initiation of Carcinogenesis During the Cell Cycle. Cell Cycle Regulation of DNA Repair Enzymes and Pathways. DNA Repair and Poly(ADP-ribose) Synthesis in Human Fibroblasts and Other Cell Types. Repair of O-Alkylation Damage in Human Cells. Ultraviolet Radiation-Induced Transformation of Human Cells. Biological Characterization and Quantitation of Markers of Transformation of Human Fibroblasts. Mechanisms for Cell Transformation and Carcinogenesis by Genotoxic and Nongenotoxic Agents: Role of Sodium Saccharin. Relationship Between Bulky Carcinogen-DNA Modification: Carcinogenesis and Mutagenesis. Metabolism of Polyaromatic Hydrocarbons by Human Fibroblasts. DNA Adducts and Human Cell Transformation. Cytotoxicity and Cell Culture. Mechanisms by Which Phorbol Ester Tumor Promoters Affect Gene Expression in Human Cells. Changes in Gene Expression Associated with Neoplastic Transformation of Human Fibroblasts: The Abundant Cellular Proteins. Differential Repair of Damage in Specific DNA Sequences of Human Cells. Index.