1st Edition

Visualization of Receptors In Situ Applications of Radioligand Binding

By Emmanuel Moyse, Slavica M Krantic Copyright 2001
204 Pages
by CRC Press

208 Pages
by CRC Press

Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures - often not... Read more
LIGHT MICROSCOPY. Ligand Labeling with Radioisotopes. Tissue Preparation for Light Microscopy. Radioligand Binding Assay on Frozen Tissue Sections. Film (Dry) Radioautography. Liquid Emulsion (Wet) Radioautography. Quantification of Radioautographic Labeling. ELECTRON MICROSCOPY. Choice of Radioligand. Tissue Preparation. Radioligand Binding Assay on Thick Tissue Slices. Embedding and Ultrathin Sectioning. Radioautography. Coloration and Contrasting of Ultrathin Sections. Analysis and Quantification of Radioautographic Labeling.

Biography

Emmanuel Moyse, Slavica M Krantic