Vitrification in Assisted Reproduction presents standard and new cryopreservation techniques in detail, outlining those that have resulted in success, and providing recommended means for overcoming typically encountered problems.
This new edition provides a much broader range of clinical application and data to demonstrate its contribution to the use of vitrified oocytes and embryos. The book also discusses new areas in the field of assisted reproductive technology such as oocyte banking, preimplantion diagnostics at the blastocyst stage, and the burgeoning adoption of elective single embryo transfer.
Written by expert scientists and clinical embryologists, this book will help you to consistently and predictably apply vitrification as an important therapeutic strategy in assisted reproduction.
Table of Contents
Overview of Biological Vitrification
Gregory M. Fahy
Vitrification of Oocytes and Embryos: Finally a Recognized Technique, But Still a Source of Concern and Debate
Pierre Vanderzwalmen, Nicolas Zech, Fabien Ectors, Yannis Panagiotidis, Achillae Papatheodorou, Prapas Yannis, and Barbara Wirleitner
Intracellular Concentration of Cryoprotectant During Vitrification and Slow-Freezing Cryopreservation Procedures?
Pierre Vanderzwalmen, Fabien Ectors, Barbara Wirleitner, Astrid Stecher, Deborah Desmet, Jacqueline Greindl, Amandine Helson, Sabine Vanderzwalmen, Mark G. Larman, Nicolas Zech, and Delphine Connan
Importance of Cooling Versus Warming Rates in Vitrification Techniques
The Movement of Water and Cryoprotectants in Mammalian Oocytes and Embryos: Membrane Permeability and Aquaporins
Keisuke Edashige and Magosaburo Kasai
Open Versus Closed Systems
Mark G. Larman and Pierre Vanderzwalmen
Automatic Vitrification: Development of the Gavi System
Tammie K. Roy, Susanna Brandi, Cara K. Bradley, and Teija T. Peura
Vitrification at Minimum Volume: From Basic Science to Clinical Application
Vitrification of Oocytes: General Considerations and the Use of the Cryotec Method
Safety of Vitrification and Cryostorage and Optimization of Cryopreservation Protocols
Lodovico Parmegiani and Marco Filicori
Physiological Aspects of Oocyte Vitrification
Mark G. Larman and David K. Gardner
Vitrification of Oocytes: Imprinting and Disturbance in Spindle Formation and Chromosome Segregation
Metabolic Profile of Day 3 Embryos Arising from Vitrified Oocytes
Damià Castelló and Francisco Domínguez
Vitrification of Human Oocytes for In Vitro Fertilization Patients
Laura Rienzi, Benedetta Iussig, and Filippo M. Ubaldi
Oocyte Vitrification: Donor "Egg Banking"
Zsolt Peter Nagy, Ana Cobo, and Ching-Chien Chang
Fertility Preservation for oncology Patients
Kara N. Goldman, Caroline McCaffrey, and Nicole Noyes
Vitrification of Human Ovarian Tissue
Mona Sheikhi and Outi Hovatta
Vitrification of Cleavage-Stage Embryos and Blastocysts and Their Neonatal Outcomes
Tetsunori Mukaida and Chikahiro Oka
Vitrification of Human Blastocysts: Clinical Realities and Neonatal Outcomes
Development and Hatching of Human Blastocysts After Vitrification and Warming
Joe Conaghan and Sergio Vaccari
Does Storage of Vitrified Blastocysts Have an Impact on Implantation Potential and Birth Rate?
Barbara Wirleitner, Nicolas H. Zech, and Pierre Vanderzwalmen
Ovarian Tissue Vitrification: Clinical Realities and Outcomes Chapter for From Basic Science to Clinical Application
Sherman Silber and Christina Usher
Vitrification of Human Testicular Tissue, Spermatogonia, and Spermatozoa
Christine Wyns, Gael Abou-Ghannam, and Jonathan Poels
Vitrification in Pluripotent Stem Cell Banking: Requirements and Technical Solutions For Large-Scale Biobanks
Julia C. Neubauer, Axel F. Beier, Frank Stracke, and Heiko Zimmermann
Scrying The Future: The ongoing Transformation of Reproductive Medicine Through Vitrification
Kevin S. Richter, James R. Graham, and Michael J. Tucker
Michael J. Tucker, PhD, HCLD, FI Biol., is scientific director at Georgia Reproductive Specialists in Atlanta, and Director of IVF laboratories at Shady Grove Fertility in the mid-Atlantic region of the USA. He earned a doctorate in reproductive physiology from the University of Birmingham in the UK, and is a fellow of The Royal Institute of Biology. Dr. Tucker pioneered the first intracytoplasmic sperm injection (ICSI) birth in the United States, the nation's first birth resulting from a testicular biopsy, the world's first birth from frozen donor eggs, and the first pregnancy in the world from immature eggs that were matured after thawing in the IVF lab. His current research interests include pre-implantation genetic diagnosis, oocyte and embryo cryopreservation, extended embryo growth and selection, and gamete/embryo micromanipulation. He has authored numerous peer review papers and chapters over the last 30years, and has served as a scientific consultant and lecturer in more than 50 clinics worldwide.
Juergen Liebermann PhD, HCLD (ABB), is certified as a High-complexity Clinical Laboratory Director through the American Board of Bioanalysis. Dr. Liebermann has been working in the field of IVF since 1995. He is the laboratory director for FCI's Chicago/River North Embryology Lab, all FCI Andrology Labs, and the Endocrinology Lab. In his role as director of the Embryology Laboratory, he works with eggs, semen and embryos performing various procedures such as in vitro fertilization, egg vitrification, semen analysis, embryo selection for treatment, intracytoplasmic sperm injection and preimplantation diagnostic and screening of human embryos. Dr. Liebermann’s postdoctoral thesis in 2004 qualified him as a university lecturer in Experimental Reproductive Medicine at the Bavarian University of Wuerzburg, Germany. He is a member of the American Society for Reproductive Medicine, the European Society of Human Reproduction and Embryology, the Society for the Study of Reproduction, and the American Board of Bioanalysis. He serves also as an ad hoc reviewer for various scientific publications and has reviewed more than 130 manuscripts for a variety of leading journals in the field of Assisted Reproductive Technology (ART).
Featured Author Profiles
Praise for the first edition:
"… a very interesting and important book in this exciting new field of cryobiology … I sincerely recommend this book for every ART laboratory."
—Acta Obstetricia et Gynecologica Scandinavica
"… the book presents a straightforward description of current vitrification technology to practitioners in human reproduction, as well as possible scenarios for future applications."