Working with DNA is a guide to the materials and methods that are used in the recombinant DNA laboratory and is designed to help the novice understand how specific laboratory techniques work, but also has sections that may stimulate the thinking of experienced researchers. It isn't a book of step-by-step protocols and recipes, but is intended to broaden and strengthen the foundation of the reader so that working with DNA becomes more natural and make them aware of the many ways that a molecular research question may be addressed.
Table of Contents
1. Understanding Recombinant DNA Techniques 2. The Recombinant DNA Laboratory 3. Solutions, Buffers, Stocks and Cocktails 4. Cloning Vectors 5. Restriction Endonucleases 6. Polymerases 7. The Polymerase Chain Reaction 8. Working with DNA: the reasons
Stan Metzenberg, Associate Professor, Department of Biology, California State University at Northridge
Stan Metzenberg obtained his Ph.D. degree in Molecular Biology at the University of Wisconsin-Madison, and was a post-doctoral fellow at the University of California-San Francisco. He is currently an Associate Professor of Biology at California State University, Northridge, where he teaches courses in biotechnology and recombinant DNA techniques. His principal research interests are in the molecular biology of infectious diseases.
"…there are problem based exercises, with the answers at the end, which could be valuable for tutorials, before practical classes or just to check that your research students are understanding the techniques that they are going to use in your laboratory." Journal of Biological Education