An Introduction to Microscopy: 1st Edition (Hardback) book cover

An Introduction to Microscopy

1st Edition

By Suzanne Bell, Keith Morris

CRC Press

180 pages | 115 B/W Illus.

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pub: 2009-10-21
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Description

Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many scientific disciplines.

Designed to serve as a primary resource for undergraduate or graduate students, An Introduction to Microscopy helps students master the foundational principles of microscopy. Intentionally concise, this text does not attempt to cover all aspects of all types of microscopy such as polarizing light and fluorescence. Instead, the authors’ intent is to provide students with the basic knowledge necessary to explore and understand these more advanced techniques. The authors draw from their own extensive backgrounds in forensic identification to explain the methods and ways in which microscopy shapes every investigation.

All nine chapters include questions and most include simple exercises related to the material covered. Numerous figures and photographs supplement the text and explain the procedures and principles introduced. A glossary is included as well as a convenient list of abbreviations, and references to more in-depth readings.

Offers a Fundamental Approach for Students in all Fields

The material assumes basic mathematics skill through algebra and a basic knowledge of fundamental chemistry and physics (essential for understanding optics). Although the authors used the high-quality microscopes found in their laboratories to produce the images found in the book, the information and methods can be applied to any type of microscope to which students have access.

Understanding the fundamentals of microscopy provides students with a relevant and marketable skill that can be readily applied in many fields, even if the students have not had significant academic training in the subject. Furthermore, by understanding various aspects of microscopy, students will begin to understand the science behind other related areas, such as spectroscopy, optics, and any number of applications involving analytical instrumentation.

Reviews

An Introduction to Microscopy provides a much needed, focused volume that is justifiably offered as a text for academic courses or a stand-alone reference for professionals … . Despite the authors’ backgrounds in forensic science, the volume is not specifically written as a forensic text, making the content applicable to any field that utilizes a microscope. … Chapter 6, Photomicrography, is a must read for anyone that uses digital imaging for research or forensic case reports.

… colleges and universities would benefit in adding An Introduction to Microscopy to their microscopy courses or any laboratory section of a course that utilizes the microscope. The organization of the book allows for the reader to flow from one chapter to the next, constantly building upon the previous chapter’s subject matter. This easy to read, well-illustrated volume with practical exercises throughout the text would be a worthwhile addition to the libraries of practitioners in the forensic sciences.

-- Christian Crowder,1 Ph.D., D-ABFA, Forensic Anthropologist, Department of Pathology, Office of Chief Medical Examiner, New York City, NY, writing in the Journal of Forensic Sciences, September 2010, Vol. 55, No. 5

Table of Contents

Light and Matter

Dual Nature of Light

Vector Notation and Wavefronts

Interactions of Light and Matter

Questions

Exercises

Fundamentals of Light and Optics

Refractive Index

Lenses

Aberrations

Image Formation

Resolution

Depth of Field

References and Further Reading

Questions

Exercises

Types of Microscopes

Main Components

Types of Microscopes

References and Further Reading

Questions

Exercises

Kohler Illumination

Description

Set Up and Technique

References and Further Reading

Questions

Exercises

Sample Preparation, Manipulation, and Micrometry

Preparing Samples: Basic Principles

Mounting Media

Micrometers and Measurements

References and Further Reading

Questions

Exercises

Photomicrography

Acquisition

Image Capture

Basic Image Processing

References and Further Reading

Questions

Exercises

Polarized Light Microscopy

Plane Polarized Light

Double Refraction and Calcite

Plane Polarized Light and Birefringent Materials

Polarizing Light Microscopes

Interference Colors and Extinction Points

Retardation Plates and Compensators

Example Applications

References and Further Reading

Questions

Exercises

Basic Crystallography

Crystal Systems

Isotropic and Anisotropic Crystals

References and Further Reading

Questions

Exercises

Chemical Microscopy

Physical and Chemical Properties

Precipitates, Solids, and Solubility

Microcrystal Methods

Polymorphs, Hydrates, and Co-Crystals

References and Further Reading

Questions

Exercises

About the Originator

Author

Suzanne Bell

Morgantwon, West Virginia, United States

Learn more about Suzanne Bell >>

Subject Categories

BISAC Subject Codes/Headings:
LAW026000
LAW / Criminal Law / General
LAW041000
LAW / Forensic Science
SCI047000
SCIENCE / Microscopes & Microscopy
SCI086000
SCIENCE / Life Sciences / General
TEC019000
TECHNOLOGY & ENGINEERING / Lasers & Photonics