Biochemistry in the Lab : A Manual for Undergraduates book cover
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Biochemistry in the Lab
A Manual for Undergraduates





ISBN 9781138589926
Published October 7, 2019 by CRC Press
202 Pages 59 B/W Illustrations

 
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Book Description

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.

Key Features:

  • Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience
  • Includes chemical biology as its foundation, teaching readers experimental methods specific to the field
  • Provides instructor experiments that are easy to prepare and execute, at comparatively low cost
  • Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry
  • Is written by an expert in the field

This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

Table of Contents

Buffers

Assays

ELISA

Protein Concentration

Salting out Proteins and other Biomolecules

A Discussion of Isoelectric Point and Effective Charge

Column Chromatography

Michaelis-Menten Kinetics

Protein Purification

Polyacrylamide Gels

in silico Biochemistry: the evolution of globins

Growing Crystals of Hemoglobin

Enzyme Inhibition

Multisubstrate Kinetics

Fluorescence and Denaturation

Fluorescence Studies of Ligand Binding

DNA Restriction Digests

Western Blotting

...
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Author(s)

Biography

Dr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution.