1st Edition
Biochemistry in the Lab A Manual for Undergraduates
Buffers
Assays
ELISA
Protein Concentration
Salting out Proteins and other Biomolecules
A Discussion of Isoelectric Point and Effective Charge
Column Chromatography
Michaelis-Menten Kinetics
Protein Purification
Polyacrylamide Gels
in silico Biochemistry: the evolution of globins
Growing Crystals of Hemoglobin
Enzyme Inhibition
Multisubstrate Kinetics
Fluorescence and Denaturation
Fluorescence Studies of Ligand Binding
DNA Restriction Digests
Western Blotting
Biography
Dr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution.
Author Lasseter (biochemistry, Christopher Newport Univ.) has written several laboratory manuals for general chemistry. This manual provides 18 chapters, each dealing with a single biochemical laboratory procedure. Several chapters end with a goal—assemble the methods and materials to use—and an optional goal—use and test materials after they are prepared. All chapters end with pre-lab and post-lab questions as well as references. Topics covered include buffers, assays, protein concentrations, ELISA (Enzyme-Linked ImmunoabSorption Assay), salting out biomolecules, isoelectric points and effective charges, column chromatography, Michaelis-Menten kinetics (biochemical reaction rates), protein purification, polyacrylamide gels and their uses, computational biochemistry (the example used is the evolution of globins), growing crystals of hemoglobin, enzyme inhibition, multisubstrate kinetics, fluorescence and denaturation, fluorescence studies of ligand binding, DNA restriction digests (i.e., determination of sites), and western blotting (combined use of ELISA and immunostaining). In the current pandemic time, applicability of any lab manual given total shutdown of academic labs followed by only partial reopening is problematic, yet resources are available for teaching science labs under these conditions. Undergraduate students of biochemistry and their instructors will appreciate this book given the appropriate conditions for use.
Summing Up: Recommended. Lower- and upper-division undergraduates. Students enrolled in two-year technical programs. Graduate students and faculty.
--R. E. Buntrock, independent scholar, CHOICE
