1st Edition

Chemical Approaches to the Synthesis of Peptides and Proteins

    Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications.
    This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.

    Amino Acids and Peptide and Protein Structure
    Amino Acid Activation and Coupling
    Protection Schemes for Peptide Synthesis
    The Synthesis of Peptides
    Solid-Phase Peptide Synthesis
    The Solid Support
    Protection Schemes
    Attachment of the First Amino Acid to the Solid Support
    Chain Elongation
    Cleavage of the Peptide from the Resin
    Examples of Protein Synthesis by Linear SPPS
    Peptide Synthesis in Solution
    Strategic Considerations
    Protection Schemes
    Chain Elongation
    Final Removal of Protecting Groups
    Special Approaches to the Synthesis of Peptides in Solution
    Examples of Peptide Synthesis in Solution
    Convergent Approaches to the Synthesis of Large Peptides and Proteins
    Convergent Solid-Phase Peptide Synthesis
    The Coupling of Protected Peptide Segments in Solution
    Coupling of Minimally Protected Peptides in Aqueous Solution
    Chemical Ligation of Peptide Segments
    Formation of Disulfide Bridges
    Peptides with Disulfide Bridges
    Protection of Cysteine in Peptide Synthesis
    Chemical Methods for the Formation of Disulfide Bridges
    Regioselective Disulfide Bridge Formation
    Peptide Libraries
    Multiple Peptide Synthesis in Parallel
    Peptide Library Techniques
    Examples of Peptide Library Synthesisi
    Each chapter contains References


    Paul Lloyd-Williams (Universitat de Barcelona, Barcelona, Spain), Fernando Albericio (Parc Cientific de Barcelona, Spain) and Ernest Giralt (Biomedical Research Institute, Barcelona, Spain)