G ProteinsTechniques of Analysis: 1st Edition (Hardback) book cover

G ProteinsTechniques of Analysis

1st Edition

Edited by David R. Manning

CRC Press

320 pages

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Hardback: 9780849333811
pub: 1999-05-19
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Description

Incorporating a bench-top format, G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility.

G Proteins: Techniques of Analysis includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors.

Including contributors from key institutions, each author offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, beneficial to students and professionals as well.

Table of Contents

Preface

Expression and Purification of G Protein a

Subunits in E. scherichia coli - M. Linder

Purification of Recombinant G Protein a and bg Subunits from Sf9 Cells-T. Kozasa

Expression and Functional Analysis of G Protein a Subunits in Mammalian Cells…-C. H. Berlot

Identification of Regulators and Targets of G Protein a Subunits Using the Yeast Two-Hybrid System-J.L. Glick, T. E. Meigs, and P. J. Casey

Functional Analysis of G Protein bg Subunits…-K. Yan, O. Kisselev, A. Pronin and N. Gautam

Immunological Techniques of Analysis-C. A. Chen and D. R. Manning

GTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins- J. Wang, Y. Tu, S. Mukhopadhyay, P. Chidiac, G. Biddlecome and E. M. Ross

Fatty Acid Acylation of G Protein a Subunits- P.B.Wedegaetner

ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin- M. J. Woolkalis

Analysis of Receptor.G Protein coupling with PTX-Risistant Analogues of G(i in subscript) and G(o in subscript)- A. Mortiz and R. Taussig

Microinjection of Antibodies Neutralizing G. Protein Function-V. J. LaMorte, J. R. Feramisco and J. L. Meinkoth

Agonist-Promoted (35S)GTPgS-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells- A.J. Barr and D. R. Manning

Methods of Sequestering bg in the Intact Cell- G. J. Della Rocca, R. J. Lefkowitz and W. J. Koch

In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies-H. Wang, X. Liu, Z. Cheng, H. Ling Li and C. C. Malbon

About the Series

Methods in Signal Transduction Series

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Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
MED071000
MEDICAL / Pharmacology
SCI086000
SCIENCE / Life Sciences / General