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G ProteinsTechniques of Analysis

Edited By

David R. Manning




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ISBN 9780849333811
Published May 19, 1999 by CRC Press
320 Pages

 
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Book Description

Incorporating a bench-top format, G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility.
G Proteins: Techniques of Analysis includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors.
Including contributors from key institutions, each author offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, beneficial to students and professionals as well.

Table of Contents

Preface
Expression and Purification of G Protein a
Subunits in E. scherichia coli - M. Linder
Purification of Recombinant G Protein a and bg Subunits from Sf9 Cells-T. Kozasa
Expression and Functional Analysis of G Protein a Subunits in Mammalian Cells...-C. H. Berlot
Identification of Regulators and Targets of G Protein a Subunits Using the Yeast Two-Hybrid System-J.L. Glick, T. E. Meigs, and P. J. Casey
Functional Analysis of G Protein bg Subunits...-K. Yan, O. Kisselev, A. Pronin and N. Gautam
Immunological Techniques of Analysis-C. A. Chen and D. R. Manning
GTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins- J. Wang, Y. Tu, S. Mukhopadhyay, P. Chidiac, G. Biddlecome and E. M. Ross
Fatty Acid Acylation of G Protein a Subunits- P.B.Wedegaetner
ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin- M. J. Woolkalis
Analysis of Receptor.G Protein coupling with PTX-Risistant Analogues of G(i in subscript) and G(o in subscript)- A. Mortiz and R. Taussig
Microinjection of Antibodies Neutralizing G. Protein Function-V. J. LaMorte, J. R. Feramisco and J. L. Meinkoth
Agonist-Promoted (35S)GTPgS-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells- A.J. Barr and D. R. Manning
Methods of Sequestering bg in the Intact Cell- G. J. Della Rocca, R. J. Lefkowitz and W. J. Koch
In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies-H. Wang, X. Liu, Z. Cheng, H. Ling Li and C. C. Malbon

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