Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book:
- Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies
- Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips
- Addresses the needs of researchers in academic and commercial environments
- Guides graduate students in designing, implementing, and evaluating experimental projects.
Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation.
Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.
Table of Contents
Strategies for Novel Research Projects and Research Grant Funding
Rapid Isolation of Specific cDNA or Gene by PCR
Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Subcloning of Genes or DNA Fragments
Non-isotopic and Isotopic DNA or RNA Sequencing
Information Superhighway and Computer Databases of Nucleic Acids & Proteins
Characterization of DNA or Genes by Southern Blot Hybridization
Gene Over-expression by Sense RNA in Mammalian System
Gene Under-expression by Antisense DNA and RNA Strategies
Analysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCR
Analysis of Gene Expression at the Proteomic Level via Western Blotting
Analysis of Protein Expression Using Immuno-fluorescence Localization
Analysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH)
Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH)
In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCR
Detection of Low Abundance mRNA
Isolation and Characterization of Genes from Genomic DNA Libraries
Mouse Stem Cells as a Model Mammalian Cell line for Gene Expression
New Strategies for Gene Knockout
Large-scale Expression and Purification of Recombinant Proteins
Quantitative Analysis of Functional Genome by Real-Time RT -PCR
High-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips)
Construction and Screening of Human Antibody Libraries Using Phage Display Technology
Down-regulation of Gene Expression in Mammalian Systems via SiRNA Technology
New Techniques for Identification of Protein Binding and Proteomic Interactions
William Wu, Ph.D., has extensive experience in both academic and industrial settings. He held various positions, including Research Assistant Professor at the University of Michigan Medical School, Ann Arbor, Michigan, USA, and Professor at the Hunan Nonnal University, Hunan, the People's Republic of China, Senior Scientist, Team-Leader and Chief Technology Officer (CTO) in biomedical and pharmaceutical companies.
Helen H. Zhang, MS., is Senior Research Associate at the University of Michigan Medical School, Ann Arbor, Michigan, USA. In 1996, she received her MS degree in Molecular and Cellular Biology at the Eastern Michigan University, Ypsilanti, Michigan, USA.
Michael J. Welsh, Ph.D., is Professor of Cell Biology in the Department of Cell and Developmental Biology at the University of Michigan Medical School, Ann Arbor, Michigan, USA. He is also a Professor of Toxicology in the School of Public Health at the University of Michigan, Ann Arbor.
Peter B. Kanfman, Ph.D., is Professor of Biology in the Plant Cellular and Molecular Biology Program in the Department of Biology and a member of the faculty of the Bioengineering Program at the University of Michigan.