Gene Silencing by RNA Interference: Technology and Application, 1st Edition (Hardback) book cover

Gene Silencing by RNA Interference

Technology and Application, 1st Edition

Edited by Muhammad Sohail

CRC Press

400 pages | 4 Color Illus. | 68 B/W Illus.

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pub: 2004-08-27
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Description

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by renowned researchers to provide the most diverse and in-depth examination of the subject yet.

This book describes methods and protocols for gene silencing and RNA interference. Each chapter provides necessary background to the subject and then gives detailed methods in easy-to-follow steps, along with troubleshooting hints and tips. Following a general and historical introduction, chapters two through eight focus on technical details of the various methods of siRNA design, chemical and enzymatic production, plasmid- and virus-mediated intracellular expression, and other RNAi tools. Chapter nine discusses the exogenous delivery of siRNAs into cells, and the final chapters of the book detail the application of RNAi to dissect gene function in a number of biological systems, including cell-free systems, cultured cells, and whole organisms.

From the design and production of RNAi tools to their applications, Gene Silencing by RNA Interference: Technology and Application is the first to present the entire spectrum of activity, design, production, and delivery of RNAi reagents, providing a welcome guide for both academic and commercial use.

Table of Contents

Gene silencing by RNA interference and the role of small interfering RNAs; N.J. Caplen, National Institutes of Health, Maryland, USA

Basics of siRNA design and chemical synthesis; Q. Boese, A. Reynolds, and W.S. Marshall, Dharmacon, Inc. Colorado, USA

Oligonucleotide scanning arrays in the design of small interfering RNAs; V.M. Macaulay and M. Sohail, Weatherall Institute of Molecular Medicine and University of Oxford, England, UK

siRNA production by in vitro transcription; O. Donzé, P. Dudek, and D. Picard, Université de Genève and Apotech Corporation, Switzerland

Production of siRNAs with the application of deoxyribozymes; G. Doran and M. Sohail, University of Oxford, England, UK

Production of siRNA in vitro by enzymatic digestion of double-stranded RNA; F. Buchholz, D. Drechsel, M. Ruer, and R. Kittler, Max Planck Institute, Dresden, Germany

Plasmid-mediated intracellular expression of siRNAs; R. Wadhwa, M. Sano, M. Miyagishi, and K. Taira, National Institute of Advanced Industrial Science and Technology, Tsukuba, and University of Tokyo, Japan

Lentiviral vector-mediated delivery of si/shRNA, D.S. An, X-F. Qin, Vincent Auyeung, D. Baltimore, and I.S.Y. Chen, University of California-Los Angeles, UT MD Anderson Cancer Center in Texas, and California Institute of Technology, Pasadena, USA

Exogenous siRNA delivery: protocols for optimizing delivery to cells; A.J. Hollins, S.P. Fox, and S. Akhtar, Cardiff University, Wales, UK

RNAi in Drosophila cell cultures, M. Bettencourt-Dias, R. Sinka, L. Frenz, and D. Glover, University of Cambridge. and Cyclacel, Ltd., England, UK

RNAi in Caenorhabditis elegans; F. Simmer and R.H.A. Plasterk, Center for Biomedical Genetics, Utrecht, The Netherlands

Delivery of RNAi reagents in C. elegans by microinjection; P. Appleford and A. Woollard, University of Oxford, England, UK

RNAi in Drosophila embryos; R.W. Williams, University of California, Berkeley, USA

RNAi in Xenopus laevis oocytes; E. Gordon and G.W. Abbott, Cornell University, New York, USA

RNAi in Xenopus embryos; K.H. Kok and D-Y. Jin, University of Hong Kong, People's Republic of China

An RNAi-based genomic library for forward genetics in the African Trypanosome; J.C. Morris, Z. Wang, S.A. Motyka, M.E. Drew, and P.T. Englund, Clemson University, South Carolina, Massachusetts Institute of Technology, Massachusetts, Johns Hopkins University, Maryland, and Washington University, Missouri, USA

RNAi in the malaria parasite Plasmodium; A. Brüning-Richardson and G.A. McConkey, University of Leeds, England, UK

Silencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches; P.V.N. Dasaradhi, A. Mohmmed, M.J. Hossain, V.S. Chauhan, and P. Malhotra, International Center for Genetic Engineering and Biotechnology, New Delhi, India

RNA in transgenic animal models; H. Hasuwa & M. Okabe, Osaka University, Japan

RNA interference in mouse model, E. Song, P. Stern, D. Palliser, Luk Van Parijs and J. Lieberman, Harvard University, Massachusetts, USA

Virus-induced RNA silencing in plants; M. Figlerowicz, M. Barciszewska-Pacak, B. Grabowska, J. Wojciechowicz, and A. Pacak, Adam Mickiewicz University, Poznan, University of Warmia and Mazury, Olsztyn, and Polish Academy of Sciences, Poznan, Poland

RNA silencing in plants - biolistic delivery of RNAi reagents; U. Klahre and F. Meins, Novartis Research Foundation, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

Agro-infiltration: a versatile tool for RNAi studies in plants; D. Silhavy, Agricultural Biotechnology Center, Godollo, Hungary

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
SCI029000
SCIENCE / Life Sciences / Genetics & Genomics
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology
SCI086000
SCIENCE / Life Sciences / General