Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles.
Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
Table of Contents
1. Basic Principles of Gene Transfer 2. Chemical and Physical Transfection 3. Cell-Mediated Gene Transfer 4. Gene Transfer using Viral Vectors 5. Genetic Manipulation of Animals 6. Gene Targeting in Animal Cells 7. Control of Transgene Expression 8. Transgene Behaviour 9. Additional Strategies for Transgene Inactivation 10. Applications of Gene Transfer
"This is an excellent book that will bring readers up to date with a broad range of experimental approaches for gene expression… The uniqueness of this volume is that it is broad in scope yet thorough in detail… This book is a must for anyone who seeks to keep abreast with this exciting endeavour." The Quarterly Review of Biology