The Advanced Methods series is intended for graduate researchers in the biological and biomedical life sciences. Titles contain both background information and detailed protocols and aim to give sufficient theory to enable users to adapt the given protocols to their own experimental system.
Molecular Microbial Ecology
Gene Transfer to Animal Cells
Cell Proliferation and Apoptosis
Gene Targeting and Embryonic Stem Cells
By Martin Latterich
December 20, 2007
One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful ...
By Ulrike Nuber
December 22, 2005
DNA Microarrays introduces all up-to-date microarray platforms and their various applications. It is written for scientists who are entering the field of DNA microarrays as well as those already familiar with the technology, but interested in new applications and methods....
By M Tevfik Dorak
June 13, 2006
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the ...
By Mark Osborn, Cindy Smith
September 29, 2005
Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of ...
By Richard Twyman
February 11, 2005
Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes ...
By David Hughes, Huseyin Mehmet
May 30, 2003
Cell Proliferation and Apoptosis provides a detailed practical guide to cell proliferation and apoptosis detection methods. A novel approach combining both these areas allows important comparisons to be made. Topics covered include all aspects of tissue handling from collection, storage, fixation ...
By Jim Mcwhir, Alison Thomson
March 01, 2004
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting ...
By Ian Day, S Ye
June 25, 2003
The 'Advanced Methods' series is intended for advanced undergraduates, postgraduates and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to ...