1st Edition

Molecular Microbial Ecology

Edited By Mark Osborn, Cindy Smith Copyright 2005
400 Pages
by Taylor & Francis

256 Pages
by Taylor & Francis

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary... Read more

1. Nucleic Acid Extraction From Environmental Samples. 2. Prokaryotic Systematics: PCR And Sequence Analysis Of Amplified 16S rRNA Genes. 3. DNA Fingerprinting Of Microbial Communities. 4. Molecular Typing Of Environmental Isolates. 5. RT-PCR And mRNA Expression Analysis Of Functional Genes. 6.Quantitative Real-Time PCR. 7. Stable Isotope Probing. 8. Applications Of Nucleic Acid Hybridization In Microbial Ecology. 9. Fluorescence in situ Hybridisation For The Detection Of Prokaryotes. 10. Lessons From The Genomes: Microbial Ecology And Genomics. 11. Metagenomic Libraries From Uncultured Microorganisms. 12. A Molecular Toolbox For Bacterial Ecologists: PCR Primers For Functional Gene Analysis. 13. Molecular Detection Of Fungal Communities In Soil. 14. Environmental Assessment: Bioreporter Systems. 15. Bioinformatics And Web Resources For The Microbial Ecologist.

Biography

Dr A. Mark Osborn-Department of Animal & Plant Sciences, University of Sheffield