362 Pages
83 B/W Illustrations
by
Taylor & Francis
333 Pages
by
Taylor & Francis
Also available as eBook on:
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and... Read more
1. An Introduction to Real-time PCR 2. Data Analysis and Reporting 3. Relative Quantification 4. Normalisation 5. High-Throughput Primer and Probe Design 6. Quantitative Analysis of Ocular Gene Expression 7. Quantitative Gene Expression by Real-time PCR 8. Real-time PCR Using SYBR Green 9. High-Resolution Melting Analysis for Scanning and Genotyping 10. Quantitative Analyses of DNA Methylation 11. Mitochondrial DNA Analysis 12. Real-time Immuno-PCR 13. Clinical Microbiology 14. Clinical Virology 15. Solid Organ Transplant Monitoring 16. Real-time PCR Applications in Haematology 17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies
Biography
Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK
The goal is to provide both theoretical and practical information for those wishing to use real time PCR as a laboratory tool. This is a generally worthwhile aim and, with some caveats, suitably met. This is a useful book in that limitations and uses are both covered. Doody's Notes, February 2007
