RNAi: 1st Edition (Paperback) book cover


1st Edition

Edited by Martin Latterich

Taylor & Francis

150 pages

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Paperback: 9780415409506
pub: 2007-11-08
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eBook (VitalSource) : 9780203967218
pub: 2008-09-04
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One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful research tool in the functional study of individual genes and their products, and in functional genomics. In RNAi, specific small double-stranded RNA molecules (small interfering RNAs or siRNAs) are introduced into cells to selectively silence certain genes.

RNAi covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent. A key feature of RNAi is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations.

Table of Contents

1. Methods in RNA Interference 2. RNAi Reagent Design 3. RNA Interference - A Chemical Perspective 4. Validation of RNAi 5. RNA Interference Libraries in Dissecting Molecular Pathways of the Human Cell 6. High-Throughput RNAi in Caenorhabditis elegans - From Molecular Phenotypes to Pathway Analysis 7. RNAi in Xenopus laevis 8. Generation of Transgenic and Knockdown Mice with Lentiviral Vectors and RNAi Techniques 9. RNAi in Fungi

About the Editor

Martin Latterich

About the Series

Advanced Methods

The Advanced Methods series is intended for graduate researchers in the biological and biomedical life sciences. Titles contain both background information and detailed protocols and aim to give sufficient theory to enable users to adapt the given protocols to their own experimental system.

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Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Cytology
SCIENCE / Life Sciences / Genetics & Genomics
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Life Sciences / General