Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail.
This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers.
- Addresses topics associated with historical development and principles of genome-editing technology
- Addresses basic mechanisms operating under each genome-editing technology
- Addresses its application in plants to design crops as per the current and future demands
- Addresses the regulatory mechanisms of genome-edited crops
Table of Contents
Historical developments of genome editing in plants
Ravindra Ramrao Kale, Prashant Raghunath Shingote, Dhiraj Lalji Wasule, Shriram Jaganath Mirajkar, Darasing Ramsing Rathod, and Mangesh Pradeep Moharil
Mechanism of ZFN Mediated Genome Editing: Scope and Opportunities
Nabaneeta Basak, Vivek Chandra Verma, Rajeev Kumar, Gaurav Kumar
TALEN: customizable molecular scissors for tailoring newer types of genomes in plants
Ravi Prakash Saini, Manjunatha N, Manoj Kumar, Pawar Deepak Viswanath
Mechanism of CRISPER/Cas9 mediated genome editing: Scope and opportunities
Antim, Ashish, Bhudev Singh Tyagi, Mamrutha HM, Sonia Sheoran, Om Prakash Gupta
Genome editing in wheat and millets
Ram Sewak Singh Tomar, Sushma Tiwari, Prabha Singh, Bhojaraja Naik K, Anil Kumar
Genome Editing for Improvement of Rice
Mayuri D. Mahalle, Suhas G. Karkute, Umakanta Ngangkham, Amolkumar U. Solanke
Genome Editing using CRISPR/Cas systems in Legumes
Shallu Thakur, Neetu Kushwah, Sudhir Kumar, Prateek Singh, Meenal Rathore, Narendra Pratap Singh, Alok Das
Genome editing in oilseed crops
Ritesh Kumar Raipuria, Archana Watts, Nand Lal Meena and Anshul Watts
Towards "CRISPrY" vegetables: status of genome editing in vegetable crops
Amit Pareek, Amolkumar U Solanke, Vikas Mangal, Gitanjali Jiwani, Mohd. Tasleem, Om Prakash Gupta, Suhas Gorakh Karkute
Genome editing in fruit tree crops: Prospects and challenges
Genome Editing for Metabolic Engineering in Plants
Sandeep Jaiswal, Ashok Kumar, Jyoti Priya, Sivalingam Anandhan, Shweta Singh, Rupesh Kumar Singh
Genome Editing in Algae: Perspectives and Challenges
Kavita Rani, Jitendra Kumar, Taruneet kaur, Vikas Kumar, Om Prakash Gupta
Plant genome editing in basic research to understand molecular functions
Jagannadham PTK, Siddanna Savadi and Senthilkumar K Muthusamy,
Genome editing in plant: Challenges and opportunities
Gaurav Kumar, Bhupendra Singh Panwar, Nabaneeta Basak
Regulatory approaches for genome edited crops
Awadhesh Kumar, Lopamudra Nayak, Chandrasekhar Sahu
Advances in Genome editing
Shweta Singh, Rupesh Kumar Singh, Sivalingam Anandhan, Jyoti Priya, Sandeep Jaiswal Ashok Kumar
Om Prakash Gupta, PhD, received his M.Sc. and Ph.D. from Chandra Shekhar Azad University of Ag. & Technology, Kanpur and ICAR-Indian Agricultural Research Institute (IARI), New Delhi, respectively. He is presently working as scientist (Plant Biochemistry) at ICAR-Indian Institute of Wheat and Barley Research (ICAR-IIWBR), Karnal.Dr. Gupta has more than 10 years of research experience in the area of Plant Biochemistry and Molecular biology and has significantly contributed in deciphering the role of small RNA during various biotic and abiotic stresses, biofortification, marker development, nutritional and processing quality etc. He has co-developed three genetic stocks and one bread wheat variety suitable for various quality traits.
Dr. Gupta has been bestowed with more than 80 publications to his credit which includes 30 research and review papers, 20 book chapters, 4 books, 30 popular articles. He presented his research papers in several national and international symposia/ workshops/ conferences. He has immensely contributed in resource generations through several externally funded projects funded by Indian agencies like DBT & DST, and International agency including CIMMYT. Dr. Gupta is also involved in teaching Fundamentals of Molecular Biology course to PhD students. Due to his immense research contributions, Dr. Gupta has been serving as editorial board members and reviewers of many international journals. Due to his outstanding contribution in research and meritorious profile, Dr. Gupta has the distinction of receiving numerous honors, fellowships and awards in recognition to his excellent academic and research contributions. He is fellow of the Society for Advancement of Wheat and Barley Research (SAWBAR), and received several awards including JawaharLal Nehru Award for outstanding Doctoral thesis by ICAR, University Silver Medal, Aspee Gold Medal and Dr. Kirtikar Memorial Gold Medal, ChowdharyCharan Singh Memorial Award during his Bachelor degree programme.
Suhas Gorakh Karkute is a scientist in Indian Council of Agricultural Research. India. He is a graduate in Agricultural Sciences from Dr. Vasantrao Naik Marathwada Agricultural University, Parbhani, Maharashtra. He has been awarded with M.Sc. in Molecular Biology and Biotechnology from a prestigious ICAR-Indian Agricultural Research Institute, New Delhi in 2010. He is the recipient of several fellowships and awards in recognition to his performance in academics and scientific career. He has also qualified number of national level competitive examinations such as ICAR-JRF, ARS, CSIR-NET, IARI-Ph.D., JNU-Biotech, IISC-Ph.D. etc. Suhas Karkute has a research experience of more than 6 years since he joined as ARS scientist (Agricultural Biotechnology) at ICAR-Indian Institute of Vegetable Research, Varanasi. He is also working as Institute Biosafety officer for transgenics projects in the institute for past 4 years. Besides, he has the experience of working as an Examiner of Patents and Designs in Patent Office, Govt. of India for nearly 2 years.
During his scientific career at ICAR-IIVR, he is actively involved in several research projects of vegetable improvement. He is working in the area of functional genomics, transgenic development and genome editing with the aim to understand the abiotic stress tolerance in plants. He is also associated with studies on plant bioactive compounds. He has published many original research and review article in peer reviewed international journals and several book chapters and popular articles. He has participated and presented his research work in different national and international conferences/workshops/seminars. He is also contributing as reviewer of many national and international journals.