Handbook of Non-Invasive Methods and the Skin: 2nd Edition (Hardback) book cover

Handbook of Non-Invasive Methods and the Skin

2nd Edition

Edited by Jorgen Serup, Gregor B. E. Jemec, Gary L. Grove

CRC Press

1,056 pages | 9 Color Illus. | 721 B/W Illus.

Purchasing Options:$ = USD
Hardback: 9780849314377
pub: 2006-02-23
SAVE ~$55.00
eBook (VitalSource) : 9780429128912
pub: 2006-02-23
from $137.50

FREE Standard Shipping!


Firmly established as the leading international reference in this field, Non-Invasive Methods and the Skin broke new ground with its comprehensive coverage of methods used in both clinical and experimental dermatology. Completely revised and updated, containing more than twice as much information, the Second Edition continues the tradition. The authors' thorough research and clear organization make this book a baseline reference for those using noninvasive biophysical methods to study the skin.

Arranged by physical modality and structured to provide educational and practical information, the second edition, like its predecessor, will prove to be of value to young researchers and senior scientists alike. The coverage of major evaluation and measurement methods share a consistent format, including scope, sources of error, application, and validity. This edition incorporates 69 revised chapters with more than 90 new chapters covering topics such as computer technique, imaging techniques, skin friction, barrier functions, and more.

New chapters provide coverage of:

  • computers, computer techniques, and image analysis

  • imaging techniques, including clinical photography

  • legal situations and guidelines behind instrumental use

  • skin friction

  • barrier functions

  • important new techniques such as in vitro confocal microscopy, OCT, and Raman spectroscopy

  • veterinary/animal research use of methods

    The truly interdisciplinary, international panel of contributors includes experts from the specialties of dermatology, bioengineering, pathology, manufacturing engineering, medical physics, pharmacology, microbiology, neurology, surgery, obstetrics and gynecology, cardiovascular research, and pharmacy from academic institutions and hospitals in countries such as Denmark, Germany, the United Kingdom, the United States, Japan, Israel, Taiwan, and Singapore. The revision is extensive and covers a broad spectrum of methods while providing the same caliber of authoritative information that made the previous edition so popular. Application oriented, practical, and instructive, this Second Edition will meet the needs of the researchers today, and in years to come.

  • Reviews

    "…If you are interested in doing basic science or clinical dermatology research, you will certainly want to have this reference….I am unaware of any other book that accomplishes what this book does…extremely valuable."

    -Doody's Reviews

    Table of Contents


    Personal Perspectives on Bioengineering and the Skin: The Successful Past and the Brilliant Future, A.M. Kligman

    How to Choose and Use Non-Invasive Methods, J. Serup

    A Practical Guide to Resources on the Internet for the Skin Researcher, E. Grove Wickersheim and G. Lee Grove

    The Skin Integument: Variation Relative to Sex, Age, Race, and Body Region, N. Farinelli and E. Berardesca

    Seasonal Variations and Environmental Influences on the Skin, C.L. Goh

    Non-Invasive Methods and Assessment of Skin Diseases, S. Seidenari, F. Giusti, and G. Pellacani

    Standards for Good Clinical Practice (GCP), M. Thyme

    Statistical Analysis of Sensitivity, Specificity, and Predictive Value of a Diagnostic Test, N. Lange and M.A. Weinstock

    Sample Size Calculation, C. Bay and S. Møller

    Implementation of a Quality Management System in a Contract Laboratory Working with Non-Invasive Methods, K.-P. Wilhelm and J. Hofmann

    Ethical Considerations, M. Noerreslet and G.B.E. Jemec


    Skin Surface, Epidermal Structure, and Function

    Clinical Photography, Surface Imaging Techniques, and Computerized Image Analysis

    General Aspects in Medical/Clinical Photography, N. Kentorp

    Use of Compact Digital Camera for Snap Photography, K.-I. O'goshi

    Computerized Image Analysis of Clinical Photos, S.S. Hawkins

    Magnifying Lens - Non-Invasive Oil Immersion Examination of the Skin, H.I. Katz and J.S. Lindholm

    Dermatoscopy, W. Westerhof

    Fiber-Optic Microscopy System for Skin Surface Imaging, I. Sadiq and T. Stoudemayer

    Automated Assessment of Pigment Distribution and Color Areas for Melanoma Diagnosis, S. Seidenari, G. Pellacani, and C. Grana

    Skin Surface Contour and Roughness Assessment

    Skin Replication for Light and Scanning Electron Microscopy, B. Forslind

    Skin Surface Replica Image Analysis of Furrows and Wrinkles, P. Corcuff and J.-L. Leveque

    Stylus Method for Skin Surface Contour Measurement, J. Gassmueller, A. Kecskés, and P. Jahn

    Laser Profilometry, J. Efsen, S. Christiansen, H.N. Hansen, and J. Keiding

    Three-Dimensional Evaluation of Skin Surface: Micro- and Macrorelief, J. Mignot

    The Morphological Tree of the Cutaneous Network of Lines, H. Zahouani and Ph. Humbert

    Comparison of Methodologies for Evaluation of Skin Surface Contour and Wrinkles: Advantages and Limitations, M. Takahashi and M. Oguri

    Skin Surface Friction

    Tribological Studies on Skin: Measurement of the Coefficient of Friction, R.K. Sivamani, G. Wu, H.I. Maibach, and N.V. Gitis

    Skin Friction Evaluation by Unidirectional Stress Using a Friction Tester, M. Egawa and M. Takahashi

    Haptic Finger, M. Tanaka

    Epidermis Structure

    Cyanoacrylate Biopsy for Cytologic Evaluation of the Epidermis, J.E. Arrese, P. Quatresooz, C. Piérard-Franchimont, and G.E. Piérard

    High-Resolution Sonography of the Epidermis In Vivo, S. El Gammal, C. El Gammal, P. Altmeyer, M. Vog, and H. Ermert

    Optical Coherence Tomography in Dermatology, P. Andersen, L. Thrane, A. Tycho, and G.B.E. Jemec

    In Vivo Reflectance Mode Confocal Microscopy in Clinical and Surgical Dermatology, S. González, Y. Gilaberte-Calzada, A. González-Rodríguez, A. Torres, A. Halpern, and M. Rajadhyaksha

    In Vivo Reflectance Mode Confocal Laser Microscopy of Melanocytic Skin Lesions, G. Pellacani and S. Seidenari

    In Vivo Confocal Microscopy of the Skin Surface Using Fluorescent Markers, S.G. Thomas and L.D. Swindle

    In Vivo Confocal Microscopy Application in Product Research and Development, T. Yamashita and M. Takahashi

    Nuclear Magnetic Resonance (NMR) Examination of the Epidermis In Vivo, B. Querleux

    Spectrophotometric Intracutaneous Imaging (SIAscopy): Method and Clinical Applications, E. Claridge, S. Cotton, M. Moncrieff, and P. Hall

    Epidermis Hydration

    Epidermal Hydration: Measurement of High-Frequency Electrical Conductance, H. Tagami

    Measurement of Epidermal Capacitance, A.O. Barel and P. Clarys

    Bioimpedance as a Non-Invasive Method for Measuring Changes in Skin, I. Nicander, P. Åberg and S. Ollmar

    Comparison of Commercial Electrical Measurement Instruments for Assessing the Hydration State of the Stratum Corneum, B. Gabard, P. Clarys, and A.O. Barel


    Methods to Determine Desquamation Rate, C. Edwards

    Application of Adhesive Techniques to Harvest Stratum Corneum Material, D.L. Miller

    Dry Skin and Scaling Evaluated by D-Squames and Image Analysis, H. Schatz, P.J. Altmeyer, and A.M. Kligman

    Barrier Functions and Gradients

    Measurement of Transepidermal Water Loss by Semiopen Systems, R.A. Tupker and J. Pinnagoda

    Measurement of Transepidermal Water Loss by Closed-Chamber Systems, J. Nuutinen

    Measurement of Transcutaneous Oxygen Tension, H. Takiwaki

    Measurement of Transcutaneous PCO 2, C.N. Nickelsen

    Skin Surface pH: Mechanism, Measurement, Importance, J. Fluhr, L. Bankova, and S. Dikstein

    The pH Gradient in the Epidermis Evaluated by Serial Tape Stripping, H. Öhman

    Techniques for Visualization of Ionic Gradation in Human Epidermis, M. Denda

    Skin Chamber Techniques, B. Zweiman

    Microdialysis Methodology for Sampling in the Skin, L. Groth, P. García Ortiz, and E. Benfeldt

    Skin Surface Microflora

    Sampling the Bacteria of the Skin, E.A. Eady

    Mapping the Fungi of the Skin, J. Faergemann

    Dermis Structure and Function

    Dermis Structure

    High-Frequency Ultrasound Examination of Skin: Introduction and Guide, J. Serup, J. Keiding, A. Fullerton, M. Gniadecka, and R. Gniadecki

    Ultrasound B-Mode Imaging and In Vivo Structure Analysis, S. Seidenari

    Ultrasound Assessment of Dermal Water and Edema In Vivo, M. Gniadecka

    Ultrasound Assessment of Skin Aging, G. Pellacani, F. Giusti, and S. Seidenari

    Ultrasound Imaging of Subcutaneous Tissue and Adjacent Structures, X. Wortsman, E.A. Holm, and G.B.E. Jemec

    Magnetic Resonance Spectroscopy of the Skin, A. Zemtsov

    Nuclear Magnetic Resonance Examination of Skin Disorders, S. El Gammal, R. Hartwig, S. Aygen, T. Bauermann, K. Hoffmann, and P. Altmeyer

    Raman Spectroscopy of Skin, H.G.M. Edwards

    Mechanical Properties

    Identification of Langer's Lines, J.C. Barbenel

    Suction Chamber Method for Measuring Skin Mechanical Properties: The Dermaflex ®, M. Gniadecka and J. Serup

    Suction Chamber Method for Measurement of Skin Mechanics: The Cutometer ®, K.-I. O'goshi

    Suction Chamber Method for Measurement of Skin Mechanics: The New Digital Version of the Cutometer, A.O. Barel, W. Courage, and P. Clarys

    Suction Chamber Method for Measurement of Skin Mechanics: The DermaLab, G.L. Grove, J. Damia, M.J. Grove, and C. Zerweck

    Twistometry Measurement of Skin Elasticity, P.G. Agache

    Levarometry, S. Dikstein and J. Fluhr

    Indentometry, S. Dikstein and J.W. Fluhr

    The Gas-Bearing Electrodynamo meter, C.W. Hargens

    Ballistometry, C.W. Hargens

    The Cutaneous Vasculature

    Skin Colour and Blood Vessels

    Colorimetry, W. Westerhof

    Quasi-L*a*b* Color Measurement from Digital Images, H.Takiwaki

    Practical Color Calibration for Dermatoscopic Images, C. Grana, G. Pellacani, and S. Seidenari

    Measurement of Erythema and Melanin Indices, H. Takiwaki

    Dynamic Capillaroscopy, H.S. Yu, C.H. Lee, and C.H. Chang

    Capillaroscopy and Videocapillaroscopy Assessment of Skin Microcirculation: Dermatological and Cosmetic Approaches, P. Humbert, J.-M. Sainthillier, S. Mac-Mary, A. Petitjean, P. Creidi, and T. Gharbi

    Blood Flow, Vasomotion and Vascular Functions

    Laser Doppler Measurement of Skin Blood Flux: Variation and Validation, A.J. Bircher

    Examination of Periodic Fluctuations in Cutaneous Blood Flow, R. Gniadecki, M. Gniadecka, and J. Serup

    Laser Doppler Flowmetry: Principles of Technology and Clinical Applications, G. Belcaro and A.N. Nicolaides

    Laser Doppler Imaging of Skin, K. Wårdell

    The Heat Wash-In and Heat Wash-Out Technique for Quantitative, Non-Invasive Measurement of Cutaneous Blood Flow Rate, P. Sejrsen and M. Midttun

    The 133 Xenon Wash-Out Technique for Quantitative Measurement of Cutaneous and Subcutaneous Blood Flow Rates, P. Sejrsen

    Evaluation of Lymph Flow, P.S. Mortimer

    Temperature and Thermoregulation

    Sensors and Handheld Devices for Surface Measurement of Skin Temperature, Roderick Thomas

    Thermal Imaging of Skin Temperature, E.F.J. Ring

    Assessment of Cutaneous Pain, L. Arendt-Nielsen

    Sweat Gland Distribution and Function

    Classical Techniques for the Localization of Sweat Glands, P. Dykes

    Micro-Sensor Mapping of Sudoral Activity and Skin Surface Hydration, J.-L. Lévêque

    Methods for the Collection of Eccrine Sweat, J.H. Barth

    Methods for the Collection of Apocrine Sweat, J.H. Barth

    Sebaceous Glands and Sebum Excretion

    The Follicular Biopsy, O. H. Mills, Jr.

    Measurement of Excreted Sebum Using Sebum-Absorbent Film and an Optical Reader: The TapeAnalyzer, D.L. Miller

    Quantification of Sebum Output Using Sebum-Absorbent Tapes (Sebutapes ® ), C. El Gammal, S. El Gammal, A. Pagnoni, and A.M. Kligman

    Optical Measurement of Sebum Excretion Using Opalescent Film Imprint: The Sebumeter ®, K.-I. O'goshi

    Gravimetric Technique for Measuring Sebum Excretion Rate (SER), W.J. Cunliffe and J.P. Taylor

    Fluorescence Photography of Sebaceous Follicles, A. Herpens, S. Schagen, and S. Scheede

    Methods for Assessment of Follicular Transport in Ex Vivo and In Vivo, J. Lademann

    Hair, Physical Properties, and Growth Rate

    Measurement of Hair Growth, J.H. Barth and D. Hugh Rushton

    Microscopy of the Hair: The T 875, U. Blume-Peytavi and C.E. Orfanos

    Photographic and Computerized Techniques for Quantification of Hair Growth, D. Van Neste

    Measurement of the Mechanical Strength of Hair, R. Randall Wickett

    Evaluating the Strength of Human Hair, S.B. Hornby

    Nail Structure and Growth

    Methods for Nail Assessment: An Overview, D. de Berker

    Measurement of Longitudinal Nail Growth, J.S. Roth and R.K. Scher

    Measurement of Nail Thickness, G.B.E. Jemec

    Image Analysis of the Nail Surface, C. Piérard-Franchimont and G.E. Piérard


    General Guidelines for Assessment of Skin Diseases, E.A. Holm and G.B.E. Jemec

    Sodium Lauryl Sulfate (SLS) Testing: ESCD Application and Reading Standards, R.A. Tupker

    Instrumental and Computer-Based Methods for Measurement of Surface Area Afflicted with Disease, C.H. Oh

    Instrumental Evaluation of Wheal-and-Flare Reactions, D. Van Neste

    Instrumental Evaluation of Occluded Patch Test Reactions, S. Seidenari, F. Giusti, and G. Pellacani

    Light Sources, Sunlight, and Radiation Dosimetry, H.C. Wulf

    Phototesting: Phototoxicity and Photoallergy, T. Horio


    Subject Categories

    BISAC Subject Codes/Headings:
    MEDICAL / Dermatology
    SCIENCE / Research & Methodology