Introduction to GC-MS Coupling: 1st Edition (Paperback) book cover

Introduction to GC-MS Coupling

1st Edition

By Stéphane Bouchonnet

CRC Press

228 pages | 179 B/W Illus.

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pub: 2013-02-20
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Description

Although GC-MS (gas chromatography–mass spectrometry) finds applications in fields as diverse as the food processing industry, medicine, pharmacology, and environmental analysis, the few works that are dedicated to this use of mass spectrometry are generally highly complex and theoretical. Emphasizing the practical aspects of GC-MS, without neglecting the fundamental theory, Introduction to GC-MS Coupling addresses both novice and experienced users of this technique. It presents GC-MS in a clear, instructive way and proposes solutions for the difficulties classically encountered by users.

The book begins with the core principles of gas chromatography and its specific uses with MS detectors. It discusses generalities of mass spectrometry, including the various types of MS detectors and insight into the vacuum necessary for efficient operation. Chapters cover the types of analyzers used in GC-MS and their functioning principles, with a focus on the commonly used quadrupolar analyzers, as well as the implementation, advantages, and limits of various modes of acquisition in GC-MS. The text also compares performance and limitations of quadrupolar analyzers.

The author includes a full chapter on quantification using GC-MS, a topic that can be puzzling for many chemists. Encouraging a critical approach to databases, he compares laboratory-made and commercial mass spectra databases, and describes a database research algorithm. The final chapter examines mass spectra interpretation, covering chemistry concepts such as inductive and mesomeric effects required to understand dissociation pathways, and presents a global strategy for mass spectra interpretation.

Reviews

"Introduction to GC-MS Coupling is a textbook for novices and experienced users, providing practical aspects of GC–MS, without neglecting the fundamental theory. It can be recommended to students, young scientists, and established researchers starting with GC–MS applications."

—Hans H. Maurer, Anal Bioanal Chem, 2013

Table of Contents

Concepts of Gas Chromatography

General Points

Instrumentation

Fast Chromatography

Multidimensional Chromatography

References

Introduction to Mass Spectrometry: General Points

Mass Spectrometer Components

Vacuum

Detectors

References

Ion Production in GC-MS: Sources

Definitions and General Points

Electron Ionization

Chemical Ionization

References

Ion Separation: Analyzers

General Points

Technical Features

Quadrupoles

Ion Traps

Time-of-Flight Analyzers

Magnetic Analyzers

References

Acquisition Modes in GC-MS

Definitions

Full Scan Analysis

SIR, SIM, and SIS Modes

Tandem Mass Spectrometry (MS/MS)

References

Comparison of Quadrupolar Analyzer Performances: Criteria for Choosing an Analyzer

Comparison Criteria

Spectral Repeatability and Autoprotonation

Response Functions and Limits of Detection

Chromatographic Profiles

Maintenance and Robustness

Choosing a Quadrupolar Analyzer

References

Quantification by GC-MS

Introduction

External Standard Quantification

Internal Standard Quantification

Method Validation

References

Spectra Databases for GC-MS

Limitations of Mass Spectra Interpretation

Spectra Databases

Research Algorithm

Results Display

Research Parameters

Databases and Mass Spectra Interpretation

Other Uses of Databases

References

Introduction to Mass Spectra Interpretation

Introduction

Chemistry Principles Fundamental to Mass Spectra Interpretation

Dissociation Mechanisms and Interpretation of Mass Spectra in Electron Ionization

Dissociation Mechanisms in Positive Chemical Ionization

Dissociation Mechanisms in Negative Chemical Ionization

Mass Spectra Interpretation Strategy

References

Index

Subject Categories

BISAC Subject Codes/Headings:
SCI013010
SCIENCE / Chemistry / Analytic
SCI043000
SCIENCE / Research & Methodology
SCI078000
SCIENCE / Spectroscopy & Spectrum Analysis