Life at the Nanoscale: Atomic Force Microscopy of Live Cells, 1st Edition (Hardback) book cover

Life at the Nanoscale

Atomic Force Microscopy of Live Cells, 1st Edition

Edited by Yves Dufrene

Jenny Stanford Publishing

460 pages | 133 Color Illus. | 21 B/W Illus.

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pub: 2011-08-29
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Description

Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

Reviews

"Atomic force microscopy (AFM) began as a topographical surface imaging technique, but its ability to measure the tiny forces experienced and exerted by cells and the molecules therein has made it an indispensible method for biological research. These forces are central to cell function, and no optical microscopy technique yet surpasses AFM methods for investigating these physical processes at the level of molecules and cells. Now, in this book devoted to the use of AFM in biology, leading scientists using these methods in their own research discuss the great variety of biological applications that have been developed and the many unique discoveries they have allowed. Accessible but informative descriptions and stunning images offer biologists an excellent introduction to this powerful but underexploited methodology. Any biologist considering the use of AFM in their own research is certain to find inspiration in this unique resource."

—Dr. Daniel Evanko, Chief Editor, Nature Methods

"Life at the Nanoscale provides a state-of-the-art overview about how atomic force microscopy (AFM) can be used to image living cells, to probe their attachment to substrates or to other cells, to measure their mechanical properties, and to assess the forces in single-molecule interactions. Experts in the field have contributed chapters that summarize their hands-on experience in concise, and hence most useful, reviews. So many insightful experiments have been achieved over the last decade, and many of them are now presented in this resourceful book, which comes timely for students and experts who have an interest in the interdisciplinary field of cell biology and in the application of tools that address single molecules."

—Prof. Andreas Engel, Case Western Reserve University, USA

Table of Contents

Observing the Nanoscale Organization of Model Biological Membranes by Atomic Force Microscopy

Pierre-Emmanuel Milhiet and Christian Le Grimellec

High-Resolution Atomic Force Microscopy of Native Membranes

Nikohy Buzhynskyy, Lu-Ning Liu, Ignacio Casuso and Simon Scheuring

Microbial Cell Imaging Using Atomic Force Microscopy

Mitchel J. Doktycz, Claretta J. Sullivan, Ninell Pollas Mortensen and David P. Allison

Resolving the High-Resolution Architecture, Assembly and Functional Repertoire of Bacterial Systems by in vitro Atomic Force Microscopy

Alexander J. Malkin

Understanding Cell Secretion and Membrane Fusion Processes on the Nanoscale Using the Atomic Force Microscope

Bhanu P. Jena

Nanophysiology of Cells, Channels and Nuclear Pores

Hermann Schillers, Hans Oberleithner and Victor Shahin

Topography and Recognition Imaging of Cells

Lilia Chtcheglova, Linda Wildling and Peter Hinterdorfer

High-Speed Atomic Force Microscopy for Dynamic Biological Imaging

Takayuki Uchihashi and Toshio Ando

Near-Field Scanning Optical Microscopy of Biological Membranes

Thomas S. van Zanten and Maria F. Garcia-Parajo

Quantifying Cell Adhesion Using Single-Cell Force Spectroscopy

Anna Taubenberger, Jens Friedrichs and Daniel J. Mutter

Probing Cellular Adhesion at the Single-Molecule Level

Félix Rico, Xiaohui Zhang and Vincent T. Moy

Mapping Membrane Proteins on Living Cells Using the Atomic Force Microscope

Atsushi Ikai and Rehana Afrin

Probing Bacterial Adhesion Using Force Spectroscopy

Terri A. Camesano

Force Spectroscopy of Mineral-Microbe Bonds

Brian H. Lower and Steven K. Lower

Single-Molecule Force Spectroscopy of Microbial Cell Envelope Proteins

Claire Verbelen, Vincent Dupres, David Alsteens, Guillaume Andre and Yves F. Dufrêne

Probing the Nanomechanical Properties of Viruses, Cells and Cellular Structures

Sandor Kasas and Giovanni Dietler

Label-Free Monitoring of Cell Signalling Processes Through AFM-Based Force Measurements

Charles M. Cuerrier, Elie Simard, Charles-Antoine Lamontagne, Julie Boucher, Yannick Miron and Michel Grandbois

Investigating Mammalian Cell Nanomechanics with Simultaneous Optical and Atomic Force Microscopy

Yaron R. Silberberg, Louise Guolla and Andrew E. Felling

The Role of Atomic Force Microscopy in Advancing Diatom Research into the Nanotechnology Era

Michael]. Higgins and Richard Wetherbee

Atomic Force Microscopy for Medicine

Shivani Sharma and James K. Gimzewski

About the Editor

Yves Dufrêne received his engineering degree in chemistry and bioindustries (in 1991) and a PhD degree in biophysical chemistry (in 1996) at the Université catholique de Louvain (UCL, Belgium). After a postdoc at the Naval Research Laboratory (Washington DC, USA), he became research associate (2000) of the Belgian National Fund for Scientific Research and lecturer in physical chemistry and nanobiotechnology at UCL. He is currently the head of the Laboratory of Chemistry of Interfaces.

Subject Categories

BISAC Subject Codes/Headings:
SCI010000
SCIENCE / Biotechnology
SCI047000
SCIENCE / Microscopes & Microscopy
SCI051000
SCIENCE / Nuclear Physics
SCI086000
SCIENCE / Life Sciences / General