Lipid-Mediated Signaling: 1st Edition (Hardback) book cover

Lipid-Mediated Signaling

1st Edition

Edited by Eric J. Murphy, Eric Murphy, Thad Rosenberger, Thad A. Rosenberger

CRC Press

452 pages | 58 B/W Illus.

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Description

As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade’s worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.

Part I describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated signaling. Part II highlights the use of mass spectrometry to ascertain the lipid content in various systems. Part III contains two chapters devoted to techniques used to determine the role of lipids in the activation of gene transcription. Part IV rounds out the book’s solid coverage by focusing on methods used to assess fatty acid uptake and metabolism.

Lipid-Mediating Signaling is an unrivaled reference for this rapidly expanding, yet resource-limited field, and it is a valuable resource for for cell biologists, biochemists, and pharmacologists, as well as for researchers studying signaling transduction.

Table of Contents

ENZYME SYSTEMS INVOLVED IN LIPID-MEDIATED SIGNALING

Methods for Measuring the Activity and Expression of Phospholipases A2

Prerna Rastogi, Janhavi Sharma, and Jane McHowat

Methods of Measuring the Activity and Expression of Phospholipases C

Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger

Methods for Measuring the Activity and Expression of Phospholipase D

Wenjuan Su and Michael A. Frohman

Methods for Measuring the Activity and Expression of Diacylglycerol Kinase

Kaoru Goto, Yasukazu Hozumi, Tomoyuki Nakano, Alberto M. Martelli, and Hisatake Kondo

Methods for Measuring Endocannabinoid Production and Expression and Activity of Enzymes Involved in the Endocannabinoid System

Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo

MASS SPECTROMETRY METHODS FOR LIPID ANALYSIS

Mass Spectrometry Methods for the Analysis of Phospholipid Molecular Species Analysis: A Shotgun Lipidomics Approach

Xianlin Han, Hua Cheng, Xuntian Jiang, and Youchun Zeng

Mass Spectrometry Methods for the Analysis of Bioactive Sphingolipids: A High-Performance Liquid Chromatography/Tandem Mass Spectrometry Approach

Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun

Mass Spectrometry Methods for the Analysis of Oxidant Stress in Biological Fluids and Tissues: Quantification of F2-Isoprostanes and F4-Neuroprostanes Using Mass Spectrometry

Ginger L. Milne and Jason D. Morrow

LIPID-MEDIATED REGULATION OF GENE EXPRESSION

Methods for Assessing the Role of Lipid-Activated Nuclear Receptors in the Regulation of Gene Transcription

Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier

Fluorescence Methods to Assess the Impact of Lipid Binding Proteins on Ligand-Mediated Activation of Gene Expression

Heather A. Hostetler, Avery L. McIntosh, Anca D. Petrescu, Huan Huang, Barbara P. Atshaves, Eric J. Murphy, Ann B. Kier, and Friedhelm Schroeder

METHODS TO ASSESS FATTY ACID AND PHOSPHOLIPID METABOLISM

Methods for Measuring Fatty Acids Using Chromatographic Techniques

Gwendolyn Barceló-Coblijn, Cameron C. Murphy, and Eric J. Murphy

Methods for Measuring Fatty Acid Uptake and Targeting in Cultured Cells and In Vivo

Paul G. Millner, Serena M. Lackman, and Eric J. Murphy

Methods for Kinetic Analysis of Fatty Acid Incorporation and Turnover In Vivo: A Steady-State Kinetic Radiotracer Approach

Dhaval P. Bhatt and Thad A. Rosenberger

About the Editor

Eric J. Murphy, University of North Dakota, Grand Forks, USA

Thad A. Rosenberger, University of North Dakota, Grand Forks, USA

About the Series

Methods in Signal Transduction Series

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
SCI017000
SCIENCE / Life Sciences / Cytology
SCI043000
SCIENCE / Research & Methodology