Lipid-Mediated Signaling  book cover
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Lipid-Mediated Signaling





ISBN 9781138113961
Published September 12, 2017 by CRC Press
452 Pages 58 B/W Illustrations

 
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Book Description

As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade’s worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.

Part I describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated signaling. Part II highlights the use of mass spectrometry to ascertain the lipid content in various systems. Part III contains two chapters devoted to techniques used to determine the role of lipids in the activation of gene transcription. Part IV rounds out the book’s solid coverage by focusing on methods used to assess fatty acid uptake and metabolism.

Lipid-Mediating Signaling is an unrivaled reference for this rapidly expanding, yet resource-limited field, and it is a valuable resource for for cell biologists, biochemists, and pharmacologists, as well as for researchers studying signaling transduction.

Table of Contents

ENZYME SYSTEMS INVOLVED IN LIPID-MEDIATED SIGNALING
Methods for Measuring the Activity and Expression of Phospholipases A2

Prerna Rastogi, Janhavi Sharma, and Jane McHowat
Methods of Measuring the Activity and Expression of Phospholipases C
Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger
Methods for Measuring the Activity and Expression of Phospholipase D
Wenjuan Su and Michael A. Frohman
Methods for Measuring the Activity and Expression of Diacylglycerol Kinase
Kaoru Goto, Yasukazu Hozumi, Tomoyuki Nakano, Alberto M. Martelli, and Hisatake Kondo
Methods for Measuring Endocannabinoid Production and Expression and Activity of Enzymes Involved in the Endocannabinoid System
Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo
MASS SPECTROMETRY METHODS FOR LIPID ANALYSIS
Mass Spectrometry Methods for the Analysis of Phospholipid Molecular Species Analysis: A Shotgun Lipidomics Approach
Xianlin Han, Hua Cheng, Xuntian Jiang, and Youchun Zeng
Mass Spectrometry Methods for the Analysis of Bioactive Sphingolipids: A High-Performance Liquid Chromatography/Tandem Mass Spectrometry Approach
Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun
Mass Spectrometry Methods for the Analysis of Oxidant Stress in Biological Fluids and Tissues: Quantification of F2-Isoprostanes and F4-Neuroprostanes Using Mass Spectrometry
Ginger L. Milne and Jason D. Morrow
LIPID-MEDIATED REGULATION OF GENE EXPRESSION
Methods for Assessing the Role of Lipid-Activated Nuclear Receptors in the Regulation of Gene Transcription
Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier
Fluorescence Methods to Assess the Impact of Lipid Binding Proteins on Ligand-Mediated Activation of Gene Expression
Heather A. Hostetler, Avery L. McIntosh, Anca D. Petrescu, Huan Huang, Barbara P. Atshaves, Eric J. Murphy, Ann B. Kier, and Friedhelm Schroeder
METHODS TO ASSESS FATTY ACID AND PHOSPHOLIPID METABOLISM
Methods for Measuring Fatty Acids Using Chromatographic Techniques
Gwendolyn Barceló-Coblijn, Cameron C. Murphy, and Eric J. Murphy
Methods for Measuring Fatty Acid Uptake and Targeting in Cultured Cells and In Vivo
Paul G. Millner, Serena M. Lackman, and Eric J. Murphy
Methods for Kinetic Analysis of Fatty Acid Incorporation and Turnover In Vivo: A Steady-State Kinetic Radiotracer Approach
Dhaval P. Bhatt and Thad A. Rosenberger

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Editor(s)

Biography

Eric J. Murphy, University of North Dakota, Grand Forks, USA
Thad A. Rosenberger, University of North Dakota, Grand Forks, USA