1st Edition

Methods in Reproductive Aquaculture Marine and Freshwater Species

Edited By Elsa Cabrita, Vanesa Robles, Paz Herraez Copyright 2009
    572 Pages 91 B/W Illustrations
    by CRC Press

    The large amount of information on fish reproduction available is not always readily accessible to all interested parties. Written to appeal to aquaculturalists, conservation managers, and scientific researchers, Methods in Reproductive Aquaculture provides an overview of available techniques and addresses ways to improve depleted stocks of endangered fish and other species.

    The book focuses on gamete quality and management, exploring new objectives and areas of application and research, and new tools to deal with some aspects in reproduction of aquatic species. It begins with an examination of basic methods and techniques for gamete extraction, mainly sperm collection and egg stripping in different species, then describes techniques of spawning stimulation in males and females. The volume then highlights sperm and egg quality evaluation and considers sperm analysis from a practical broodstock management point of view and in the selection of appropriate breeders. The book goes on to discuss techniques used for artificial fertilization and the procedures for obtaining modified offspring. It introduces cryopreservation procedures for sperm, oocytes, and embryos, then discusses the materials and facilities required for gamete preparation and freezing-thawing procedures. The book concludes with an extensive section detailing sperm cryopreservation protocols for fifty-six marine and freshwater species.

    Unique in its focus on both marine and freshwater aquaculture of fishes, the book provides information on endangered, highly profitable species for aquaculture or fisheries and species with high potential in laboratory research. Presenting the available information in an easily understandable way, this is not merely a techniques book, but also a complete guide to fish reproduction, gamete quality, and management.


    Reproduction and control of ovulation, spermiation and spawning in cultured fish, E. Mañanós, N. Duncan, and C. Mylonas

    Methods for sperm collection, J-C. Gwo


    Sperm quality assessment, E. Cabrita, V. Robles, and P. Herráez

    Egg quality determination in teleost fish, F. Lahnsteiner, F. Soares, L. Ribeiro, and M.T. Dinis


    Artificial fertilization in aquaculture species: from normal practice to chromosome manipulation, B. Urbányi , Á. Horváth, and Z. Bokor

    SPERM AND EGG CRYOPRESERVATIONChilled storage of sperm and eggs, J. Bobe and C. Labbé

    Basic principles of fish spermatozoa cryopreservation, J. Cloud and S. Patton

    Cryopreservation of fish oocytes, T. Zhang and E. Lubzens

    Embryo cryopreservation: what we know until now, V. Robles, E. Cabrita, J.P. Acker, and P. Herráez

    Cryobiological material and handling procedures, F. Martínez-Pastor and S.L. Adams


    Cryopreservation of sperm of loach (Misgurnus fossilis), J. Kopeika, E. Kopeika, T. Zhang, and D. Rawson

    Zebrafish Sperm Cryopreservation with N, N-Dimethylacetamide, J.P. Morris IV, A. Hagen, and J.P. Kanki

    Sperm cryopreservation of live-bearing fishes of the Genus Xiphophorus, Q. Dong, C. Huang, L. Hazlewood, R.B. Walter, and T.R. Tiersch

    Use of post-thaw silver carp (Hypophtalmichthys molitrix) spermatozoa to increase hatchery productions, B. Alvarez, A. Arenal, R. Fuentes, R. Pimentel, Z. Abad, and E. Pimentel

    Cryopreservation of common carp sperm, B. Urbányi and Á. Horváth

    Sperm cryopreservation from sea perch, Lateolabrax japonicas, S-L. Chen, X.S. Ji, and Y-S. Tian

    Semen cryopreservation of piracanjuba (Brycon orbignyanus), an endangered Brazilian species, A.T.M. Viveiros and A.N. Maria

    Cryopreservation of endangered Formosan landlocked salmon (Oncorhynchus masou formosanus) semen, J-C. Gwo, H. Ohta, K. Okuzawa, L-Y. Liao, and Y-F. Lin

    Protocols for the cryopreservation of Salmonidae semen, Lota lota (Gadidae) and Esox lucius (Esocidae), F. Lahnsteiner and N. Mansour

    Rainbow trout (Oncorhynchus mykiss) sperm cryopreservation, P. Herráez, V. Robles, and E. Cabrita

    Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss), V. Robles, E. Cabrita, and P. Herráez

    Cryopreservation of testicular sperm from European catfish (Silurus glanis), G. Maisse, B. Ogier de Baulny, and C. Labbé

    Semen Cryopreservation of the African catfish, Clarias gariepinus, A.T.M. Viveiros and J. Komen

    Cryopreservation of sperm from species of the order Acipenseriformes, Á. Horváth, B. Urbányi, and S.D. Mims

    Different protocols for the cryopreservation of European eel (Anguilla anguilla) sperm, J.F. Asturiano

    Cryopreservation of Striped Bass Morone saxatilis Spermatozoa, L.C.Woods III, S. He, and K. Jenkins-Keeran

    Cryopreservation of semen from striped trumpeter (Latris lineata), A.J. Ritar

    Cryopreservation of Atlantic halibut sperm, Hippoglossus hippoglossus, I. Babiak

    Sperm cryopreservation: an actual tool for Seabass (Dicentrarchus labrax) reproduction and breeding control in research and production, C. Fauvel and M. Suquet

    Notes on Diplodus puntazzo sperm cryopreservation, F. Barbato, S. Canese, F. Moretti, A. Taddei, A. Fausto, L. Abelli, M. Mazzini, and K.J. Rana

    Sperm Cryopreservation from the Marine Teleost, Sparus aurata, E. Cabrita, V. Robles, C. Sarasquete, and P. Herráez

    Cryopreservation of seabream semen, J-C. Gwo

    Cryopreservation of sperm from winter flounder, Pseudopleuronectes americanus, R.M. Rideout, I.A.E. Butts, and M.K. Litvak

    Cryopreservation of sperm in turbot (Psetta maxima), M. Suquet, O. Chereguini, and C. Fauvel

    Cryopreservation of grouper semen, J-C. Gwo and H. Ohta

    Cryopreservation of sperm from Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus), R.M. Rideout and D.L. Berlinsky

    Cryopreservation of small abalone (Haliotis diversicolor supertexa) semen, J-C. Gwo

    Cryopreservation of Pacific Oyster Sperm, Q. Dong, C. Huang, B. Eudeline, and T. R. Tiersch

    A simple method for freezing Pacific oyster (Crassostrea gigas) sperm in quantities suitable for commercial hatcheries, S.L. Adams, J.F. Smith, R.D. Roberts, A.R. Janke, H.F. Kaspar, H.R. Tervit, P.A. Pugh, S.C. Webb, and N.G. King

    Cryopreservation of Eastern Oyster Sperm, C.G. Paniagua-Chavez and T.R. Tiersch

    Cryopreservation of sea urchin (Evechinus chloroticus) sperm, S.L. Adams, P.A. Hessian, and P.V. Mladenov

    Protocol for cryopreservation of Penaeus vannamei sperm cells, M. Salazar, M.Lezcano, and C.Granja

    Techniques for cryopreservation of spermatophores of the giant freshwater prawn, Macrobrachium rosenbergii (de Man), P. Damrongphol and K. Akarasanon

    Protocol development for the cryopreservation of spermatozoa and spermatophores of the mud crab, Scylla serrata, S. Bhavanishankar and T. Subramoniam



    Elsa Cabrita, Vanesa Robles, Paz Herraez