Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.
1. Yeast Transformation 2. Yeast Plasmids: The 2 �m Plasmid of Saccharomyces cerevisiae 3. Yeast Plasmids: Linear and Circular Plasmids from Kluyveromyces 4. Transposable Elements in Yeast 5. Transcriptional Control of Gene Expression 6. Regulation of the Galactose Genes 7. Protein Localization and Processing in Yeast: Nuclear Targeting 8. Protein Localization and Processing in Yeast: Vacuolar Protein Targeting 9. Protein Localization and Processing in Yeast: Targeting Proteins into Mitochondria 10. Protein Localization and Processing in Yeast: Peroxisomes 11. Protein Localization and Processing in Yeast: Targeting of Integral Membrane Proteins 12. Protein Secretion 13. Saccharomyces cerevisiae Mating Pheromones 14. The Killer Toxin of Saccharomyces cerevisiae 15. Saccharomyces cerevisiae Invertase 16. Saccharomyces cerevisiae Acid Phosphatase 17. The Ubiquitin System and Its Role in the Genetic Control of Proteins Half-Life 18. Heterologous Expression and Secretion