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Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites.
The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens.
This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes:
- A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group
- An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols
- A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens
This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.
Introductory Remarks
Dongyou Liu
Section I Sarcomastigophora
Acanthamoeba
Hélene Yera, Muriel Cornet, and Jean-Louis Bourges
Balamuthia
Govinda S. Visvesvara and Yvonne Qvarnstrom
Blastocystis
Hisao Yoshikawa
Dientamoeba
Damien Stark
Entamoeba
Damien Stark and John Ellis
Giardia
Yaoyu Feng and Lihua Xiao
Leishmania
Clotilde Marín, Silvia S. Longoni, and Manuel Sánchez-Moreno
Naegleria
Mineko Shibayama, Jesús Serrano-Luna, Isaac Cervantes-Sandoval, and Víctor Tsutsumi
Pentatrichomonas
Tania Crucitti
Trichomonas
Dongyou Liu, Xiangang Kong, and Changyou Xia
Trypanosoma
Emily R. Adams
Section II Ciliophora and Apicomplex
Babesia
Dongyou Liu and Xuedong Lu
Balantidium
Dongyou Liu
Cryptosporidium
Una M. Ryan and Rongchang Yang
Cyclospora
Dongyou Liu
Isospora
Chaturong Putaporntip and Somchai Jongwutiwes
Plasmodium
Dongyou Liu and Yi-Wei Tang
Sarcocystis
L. Vangeel, K. Houf, P. Geldhof, J. Vercruysse, R. Ducatelle, and K. Chiers
Toxoplasma
Kjetil Asbakk and Kristin Wear Prestrud
Section III Platyhelminthes: Cestoda
Diphyllobothrium
Barbara Wicht, Raffaele Peduzzi, Hélene Yera, and Jean Dupouy-Camet
Dipylidium
Dongyou Liu
Echinococcus and Echinococcosis
Akira Ito, Tetsuya Yanagida, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Jenny Knapp, and Yuji Ishikawa
Hymenolepis
B.R. Mirdha and Shabeena Rehman
Mesocestoides
Kerry A. Padgett, Paul R. Crosbie, and Walter M. Boyce
Spirometra
Dongyou Liu, Jianshun Chen, and Weihuan Fang
Taenia
Munehiro Okamoto and Akira Ito
Section IV Platyhelminthes: Trematoda
Clonorchis
Heinz Mehlhorn, Boris Müller, and Jürgen Schmidt
Dicrocoelium
Donato Traversa, Vincenzo Lorusso, and Domenico Otranto
Echinostomes
Rafael Toledo and Bernard Fried
Fasciola
Dongyou Liu and Xing-Quan Zhu
Fasciolopsis
Veena Tandon, B. Roy, and P.K. Prasad
Gastrodiscoides
Dongyou Liu
Haplorchis
Urusa Thaenkham and Jitra Waikagul
Heterophyidae
Ron Dzikowski and Michael G. Levy
Metagonimus
Jae-Ran Yu and Jong-Yil Chai
Nanophyetus
Dongyou Liu
Opisthorchis
Paiboon Sithithaworn, Thewarach Laha, and Ross H. Andrews
Paragonimus
Hiromu Sugiyama, Takhellambam Shantikumar Singh, and Achariya Rangsiruji
Plagiorchis
Sun Huh
Schistosoma and Schistosomiasis
Antonio Muro and Luís Pérez del Villar
Trichobilharzia
P. Horák, F.M. Schets, L. Kolárˇová, and S.V. Brant
Section V Nematoda
Ancylostoma
Jaco J. Verweij
Angiostrongylus
Dongyou Liu
Anisakis
Stefano D’Amelio, Serena Cavallero, Marina Busi, Sofia Ingrosso, Elisabetta Giuffra, Sarra Farjallah, and Graça Costa
Ascaris
Dwight D. Bowman and Janice L. Liotta
Baylisascaris
Dongyou Liu
Brugia
Kosum Chansiri and Supatra Areekit
Capillaria
Dongyou Liu
Dioctophyme
Dongyou Liu
Dirofilaria
Andrea Gustinelli, Maria Letizia Fioravanti, Monica Caffara, Lorella Garagnani, and Francesco Rivasi
Dracunculus
Dongyou Liu
Enterobius
Dongyou Liu
Gnathostoma
Paron Dekumyoy, Tippayarat Yoonuan, and Jitra Waikagul
Haemonchus
Michael B. Hildreth and Aaron Harmon
Loa
Fousseyni S. Touré Ndouo
Mansonella
Ramiro Morales-Hojas
Marshallagia
Jesús M. Pérez and Luca Rossi
Necator
Jan M. Schwenkenbecher
Oesophagostomum
Jaco J. Verweij
Onchocerca
Ramiro Morales-Hojas
Pseudoterranova and Contracaecum
Simonetta Mattiucci, Michela Paoletti, Stephen C. Webb, and Giuseppe Nascetti
Strongyloides
Dongyou Liu
Syphacia
Sanford H. Feldman and John H. Porter
Thelazia
Domenico Otranto and Donato Traversa
Toxocara
Anna Borecka
Trichinella
Edoardo Pozio and Giuseppe La Rosa
Trichostrongylus
Tai-Soon Yong
Trichuris
C. Cutillas, M. de Rojas, and R. Callejón
Wuchereria
Dongyou Liu and Frank W. Austin
Section VI Arthropoda: Arachnida
Argasidae (Soft Ticks)
Dongyou Liu
Demodex (Hair Follicle Mite)
Dongyou Liu
Dermanyssus, Ornithonyssus, and Trombicula (red, fowl, and harvest mites)
Dongyou Liu
Ixodidae (Hard Ticks)
Dongyou Liu and Chong Yin
Pyroglyphidae and Glycyphagidae (House Dust and Storage Mites)
Dongyou Liu
Sarcoptes (Itch Mite)
Dongyou Liu
Section VII Arthropoda: Insecta
Calliphoridae, Oestridae, and Sarcophagidae (Myiasis-Causing Flies)
Dongyou Liu
Cimex (Bedbug)
Dongyou Liu
Pediculus (Body and Head Lice)
Dongyou Liu and Sacha Stelzer-Braid
Phthirus (Crab Louse)
Dongyou Liu
Pulex, Xenopsylla, and Ctenocephalides (Human, Rat and Dog/Cat Fleas)
Dongyou Liu
Tunga (Jigger Flea)
Dongyou Liu
Section VIII Arthropoda: Pentastomida
Armillifer, Linguatula, and Porocephalus (Tongue Worms)
Dongyou Liu
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