2nd Edition

Official Methods for Determination of trans Fat, Second Edition

Edited By Magdi M. Mossoba, John Kramer Copyright 2010
    63 Pages
    by AOCS Publishing

    This monograph describes the most common gas chromatographic and infrared spectroscopic official methods required for the determination of trans fatty acids for food labeling purposes. The authors review the current status and limitations of the latest methods and discuss the numerous factors that may have an impact on accuracy and precision. The extensive discussion informs current researchers and analysts in nutrition and food chemistry and technology and should provide a stimulus for improving trans fatty acid methodologies.


    Structure and Occurrence of trans Fatty Acids

    Industrially Produced trans Fatty Acids

    Trans Fatty Acids Derived From Ruminants

    Trans Fatty Acids in Humans

    Capillary Gas Chromatography

    Selection of Fatty Acid Derivative

    Selection of a Catalyst for Derivatization of Lipids

    Selection of GC Column and Column Length

    Limitation of 100 m GC Columns

    Differences Between 100 m Cyanopropyl

    Polysiloxane GC Columns

    Effect of Carrier Gas

    Effect of Sample Load

    Complementary Silver-Ion Separations of

    Geometric Isomers

    Resolution of trans Isomers Other Than 18:1

    Separation of CLA Isomers Using GC and the

    Complementary Ag-HPLC Techniques

    GC Standards for FAME Identification

    Quantitation of Fatty Acid Methyl Esters by GC


    Official Methods

    Ce 1h-05, Ce 1i-07 and Related Methods

    Fourier-Transform Infrared Spectroscopy


    Transmission Mode

    Attenuated Total Reflection Mode

    Trans Fat Infrared Methodology

    Transmission FTIR Official Method AOCS

    Cd 14-95/AOAC 965.34

    ATR-FTIR Official Method AOCS Cd 14d-99/

    AOAC 2000.10

    Negative Second Derivative ATR-FTIR Methodology

    Present Status and Limitations of Official Methods


    Magdi Mossoba and John Kramer