Official Methods for Determination of trans Fat, Second Edition  book cover
2nd Edition

Official Methods for Determination of trans Fat, Second Edition

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ISBN 9781893997721
Published January 27, 2010 by AOCS Publishing
63 Pages

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Book Description

This monograph describes the most common gas chromatographic and infrared spectroscopic official methods required for the determination of trans fatty acids for food labeling purposes. The authors review the current status and limitations of the latest methods and discuss the numerous factors that may have an impact on accuracy and precision. The extensive discussion informs current researchers and analysts in nutrition and food chemistry and technology and should provide a stimulus for improving trans fatty acid methodologies.

Table of Contents


Structure and Occurrence of trans Fatty Acids

Industrially Produced trans Fatty Acids

Trans Fatty Acids Derived From Ruminants

Trans Fatty Acids in Humans

Capillary Gas Chromatography

Selection of Fatty Acid Derivative

Selection of a Catalyst for Derivatization of Lipids

Selection of GC Column and Column Length

Limitation of 100 m GC Columns

Differences Between 100 m Cyanopropyl

Polysiloxane GC Columns

Effect of Carrier Gas

Effect of Sample Load

Complementary Silver-Ion Separations of

Geometric Isomers

Resolution of trans Isomers Other Than 18:1

Separation of CLA Isomers Using GC and the

Complementary Ag-HPLC Techniques

GC Standards for FAME Identification

Quantitation of Fatty Acid Methyl Esters by GC


Official Methods

Ce 1h-05, Ce 1i-07 and Related Methods

Fourier-Transform Infrared Spectroscopy


Transmission Mode

Attenuated Total Reflection Mode

Trans Fat Infrared Methodology

Transmission FTIR Official Method AOCS

Cd 14-95/AOAC 965.34

ATR-FTIR Official Method AOCS Cd 14d-99/

AOAC 2000.10

Negative Second Derivative ATR-FTIR Methodology

Present Status and Limitations of Official Methods

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Magdi Mossoba and John Kramer