1st Edition

Opsin-free Optogenetics Technology and Applications

Edited By Yubin Zhou, Kai Zhang Copyright 2023
    202 Pages 71 Color Illustrations
    by CRC Press

    Optogenetics represents a breakthrough technology capable of dynamically modulating molecular and cellular activity in live cells with high precision. This transformative technology made it possible to reversibly interrogate protein actions, cellular events, and animal behaviors with a simple flash of light. Manipulating the temporal and spatial profile of light enabled precise control of membrane potentials. This feature has inspired non-opsin-based optogenetics, which not only inherits the precise spatiotemporal resolution but also expands the targets into a diverse pool of biomolecules such as membrane receptors, ion channels, kinases, GTPases, and transcription factors. This book is unique because it emphasizes the design and applications of opsin-free optogenetic tools.


    Key Features

    • Describes new developments in non-opsin-based optogenetic tools
    • Introduces cutting-edge methods for precise modulation of cell signaling
    • Highlights applications of optogenetic regulation of cellular functions
    • Covers contributions from an international team of leading experts

    Related Titles

      • Lim, W. & B. Mayer – Cell Signaling (ISBN 9780815342441)
      • Ayyanathan, K., ed. – Cancer Cell Signaling: Targeting Signaling Pathways Toward Therapeutic Approaches to Cancer (ISBN 9781774630839)
      • Zhang , J. et al., eds. – Optical Probes in Biology (ISBN 9781138199934)

    Table of Content

    Chapter 1

    Optogenetic dissection of a two-component calcium influx pathway

    Xiaoxuan Liu, Yingshan Wang, Yubin Zhou, and Guolin Ma*

    Chapter 2

    High-throughput engineering of a light-activatable Ca2+ channel

    Lian He*, Liuqing Wang, and Youjun Wang*

    Chapter 3

    Optogenetic activation of TrkB signaling

    Peiyuan Huang, Zhihao Zhao, Lei Lei, and Liting Duan*

    Chapter 4

    Spatiotemporal modulation of neural repair

    Huaxun Fan, Qin Wang, Kai Zhang*, and Yuanquan Song*

    Chapter 5

    Optogenetic control of neural stem cell differentiation

    Yixun Su, Taida Huang, Kai Zhang, and Chenju Yi*

    Chapter 6

    An optogenetic toolbox for remote control of programmed cell death

    Ningxia Zhang and Ji Jing*

    Chapter 7

    Control of protein levels in Saccharomyces cerevisiae by optogenetic modules that act on protein synthesis and stability

    Sophia Hasenjäger, Jonathan Trauth, and Christof Taxis*

    Chapter 8

    Optogenetics as a tool to study neurodegeneration and signal transduction

    Prabhat Tiwari* and Nicholas S. Tolwinski*

    Chapter 9

    Opsin-free optogenetics: brain and beyond

    Jongryul Hong, Yeonji Jeong, and Won Do Heo*

    Chapter 10

    Constructing a far-red light-induced split-Cre recombinase system for controllable genome engineering

    Meiyan Wang, Jiali Wu, and Haifeng Ye*

    Chapter 11

    Tools and Technologies for Wireless and Non-invasive Optogenetics

    Guangfu Wu, Vagif Abdulla, Yiyuan Yang, Michael Schneider, and Yi Zhang*


    Yubin Zhou, Kai Zhang

    “This book differs markedly from these other, more general volumes by virtue of its concentration on next generation, non-opsin photo-switches. The result is a focused, highly informative, yet compact and easy-to-read volume that offers a model for how these works should be constructed.”

    Doody Review Peter J. Kennelly, PhD (Virginia Tech)