Paramecium: Genetics and Epigenetics, 1st Edition (Hardback) book cover

Paramecium

Genetics and Epigenetics, 1st Edition

By Geoffrey Beale, John R. Preer, Jr.

CRC Press

216 pages | 32 B/W Illus.

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Hardback: 9780415257855
pub: 2008-03-27
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Description

The techniques used to decipher the genetic makeup of species as well as epigenetic mechanisms are essential for explaining life forms and studying their DNA. As a eukaryotic model, Paramecium is well suited for genetic analysis. Taking a rather unconventional view of genetics, Paramecium: Genetics and Epigenetics explores how to use this protozoan as a basis for studying complex cells.

The book discusses various aspects of Paramecium, including the cortex, the cytoplasm, nuclei, asexual fission, conjugation, autogamy,macronuclear regeneration, cytogamy, life cycle phases, and behavior. It examines the assorted mating types of the genus and how these mating types are determined. It also elucidates some techniques that identify genetically defined genes with the DNA from a library that comprises those genes and details the genetic, epigenetic, chemical, and molecular facets of several different traits. In addition, the authors chronicle the history and reemergence of investigating RNA and DNA in Paramecium.

With many powerful tools now available, Paramecium research is entering a new frontier in molecular biology. A full account of Paramecium genetics, this book presents a wealth of time-consuming observations and remarkable phenomena that will lead to a better understanding of complex cells.

Reviews

"This book has been long awaited . . . not a mere history or a collection of scientific results, but a dynamic and realistic overview because the authors themselves have been involved in most of the work . . . an excellent textbook . . ."

– Terue Harumoto, Biological Science, Nara Women’s University, in The Quarterly Review of Biology, June 2009, Vol. 84, No. 2

"In this excellent and concise book, the pre-eminent authors provide an overview of the 70 years of research on genetics and epigenetic phenomena and mechanisms in a model eukaryote, Paramecium . . . The format of the book with clearly defined chapters and subheadings in conjunction with well- presented tables, diagrams and a comprehensive index enables the reader to easily identify areas of interest . . . will be very useful for students and researchers alike."

– Jun Gong, Natural History Museum, London, in Microbiology Today, Nov 2008

Table of Contents

Foreword

Introduction

Introduction

Genetics

Paramecium

The Early Workers

Discarded Hypotheses

Epigenetics

Conclusions

Mating Types in Paramecium

Mating Types and the Species Problem in Ciliates

General Description of the Protista and Paramecium

Introduction

Morphology

Reproduction

The Life Cycle

Methods of Studying Genetic Processes in Organisms of the Paramecium aurelia Species Group

Isolation of Cells and Preparation of Pure Clones

Obtaining Complementary Mating Types

Mendelian Transmission

Caryonidal Transmission

Cytoplasmic Transmission

Methods of Identifying Genetically Defined Genes with Their DNA

The Determination of Mating Types in Paramecium

Introduction

Caryonidal Inheritance

The Cytoplasmic Effect

Independence of the Cytoplasmic Effect and the Phenotype

The O* Phenotype: A New Cytoplasmic State

The Pure E Mutant

Micronuclear Determination

Selfers

Mating Types in Species Other Than Those in the P. aurelia Complex

A Hypothesis to Explain Mating Type Inheritance

Symbionts and Mitochondria of Paramecium

Introduction

The Plasmagene Hypothesis

The Kinds of Symbionts

The Metagon

Mitochondria

Conclusions

Determination of I-Antigens

Introduction

The Immobilization Test and Serotypes

The Genetic System of I-Antigens

Chemistry and Molecular Biology of I-Antigens

Control of Expression of I-Antigen Genes

Conclusions

Ploidy Levels

Activity of Micronuclei and Macronuclei

Programmed Chromosomal Breaks and Telomeres

Internal Eliminated Sequences

Induced Mendelian Deletions

Maintaining Genic Balance at Macronuclear Division

The Paramecium Genome Project

Nuclear Dualism

Ribosomal RNA and DNA

The Early Work of Findly and Gall

More Recent Studies

Micronuclear DNA and a Model

Variants

Cortical Morphogenesis

Double Animals

Cortical Mutants

Trichocysts

Behavior

Introduction

Mutants

Genetics

Cell Models

Measuring the Electric Properties of the Cell Membrane

The Avoiding Reaction

Ion Channels

Behavioral Responses Other Than the Avoiding Reaction

Conclusions

Epigenetics

Symbionts and Mitochondria

Serotype Inheritance

Mating Type Inheritance

Cortical Inheritance

Homology-Dependent Inheritance

Conclusions

Index

References appear at the end of each chapter.

Subject Categories

BISAC Subject Codes/Headings:
SCI029000
SCIENCE / Life Sciences / Genetics & Genomics
SCI045000
SCIENCE / Life Sciences / Biology / Microbiology
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology