Anaerobic oxidation of methane (AOM) coupled to sulfate reduction (AOM-SR) is a biological process mediated by anaerobic methanotrophs (ANME) and sulfate reducing bacteria. It has scientifi c and societal relevance in regulating the global carbon cycle and biotechnological application for treating sulfate-rich wastewater. This research aimed to enhance the recent knowledge on ANME distribution and its enrichment in different bioreactor confi gurations, i.e. membrane bioreactor (MBR), biotrickling fi lter (BTF) and high pressure bioreactor (HPB). Marine sediment from Ginsburg mud volcano, Gulf of Cadiz was used as inoculum in the BTF and MBR. The BTF operation showed the enrichment of ANME in the biofi lm, especially ANME-1 (40%) and ANME-2 (10%). Whereas, the dominancy of ANME-2 and Desulfosarcina aggregates was observed in the MBR. Moreover, HPB study was performed by using highly enriched ANME-2 community from Captain Arutyunov mud volcano. During the study of HPB at different temperature and pressure conditions, the incubation at 10 MPa pressure and 15˚C was observed to be the most suitable condition for the studied AOM-SR community. Furthermore, AOM-SR activity in the coastal sediments from marine Lake Grevelingen (the Netherlands) was explored and the microbial community was characterised which was dominated by ANME-3 among known ANME types.
1. General introduction and outline
2. Physiology and distribution of anaerobic oxidation of methane by archaeal methanotrophs
3. Microbial sulfate reducing activities in anoxic sediment from Marine Lake Grevelingen
4. Anaerobic methane oxidizing microbial community in a coastal marine sediment: anaerobic methanotrophy dominated by ANME-3
5. Enrichment of anaerobic methane oxidizing ANME-1 from Ginsburg Mud Volcano (Gulf of Cadiz) sediment in a biotrickling filter
6. Enrichment of ANME-2 dominated anaerobic oxidation of methane coupled to sulfate reduction consortia from cold seep sediment (Ginsburg Mud Volcano, Gulf of Cadiz) in a membrane bioreactor
7. Response of highly enriched ANME-2a community to different pressure and temperature conditions.
8. General discussion