Plant Propagation Concepts and Laboratory Exercises  book cover
2nd Edition

Plant Propagation Concepts and Laboratory Exercises

ISBN 9781466503878
Published September 3, 2014 by CRC Press
514 Pages 340 Color Illustrations

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Book Description

Includes a DVD Containing All Figures and Supplemental Images in PowerPoint

This new edition of Plant Propagation Concepts and Laboratory Exercises presents a robust view of modern plant propagation practices such as vegetable grafting and micropropagation. Along with foundation knowledge in anatomy and plant physiology, the book takes a look into the future and how cutting edge research may impact plant propagation practices. The book emphasizes the principles of plant propagation applied in both temperate and tropical environments. In addition to presenting the fundamentals, the book features protocols and practices that students can apply in both laboratory and field experiences.

The book shows readers how to choose the best methods for plant propagation including proper media and containers as well as performing techniques such as budding, cutting, layering, grafting, and cloning. It also discusses how to recognize and cope with various propagation challenges. Also included are concept chapters highlighting key information, laboratory exercises, anticipated laboratory results, stimulating questions, and a DVD containing all the figures in the book as well as some supplemental images.

Table of Contents


Introduction to Plant Propagation, Caula A. Beyl and Robert N. Trigiano


A Brief Introduction to Plant Anatomy and Morphology, Robert N. Trigiano, Jennifer A. Franklin, and Dennis J. Gray

Plant Physiology Concepts Important for Propagation Success, Caula A. Beyl and Govind C. Sharma

Plant Growth Substances Used in Propagation, Caula A. Beyl, David W. Burger, and Zong-Ming Cheng

Sexual Reproduction and Breeding, Timothy A. Rinehart, Robert N. Trigiano, Phillip A. Wadl, and Haley S. Smith

Juvenility and Its Effect on Macropropagation and Micropropagation, Caula A. Beyl

Chimeras, Robert M. Skirvin and Margaret A. Norton


Propagation Structures: Types and Management, Gerald L. Klingaman

Intermittent Mist Control for Plant Propagation, David W. Burger

Substrates and Containers for Seed and Cutting Propagation and Transplanting, Neil S. Mattson and Amy Fulcher


Disease Management, Alan S. Windham

Botrytis and Other Propagation Pathogens, Mark P. McQuilken

Disinfestation of Soil and Planting Media, James J. Stapleton

Crop Certification Programs and the Production of Specific Pathogen-Free Plants, Danielle J. Donnelly and Adam Dale

Building a Plant Propagator’s Integrated Pest Management Toolkit, William E. Klingeman


Evaluation of Data from Propagation Experiments, Michael E. Compton


Cloning Plants by Rooting Stem Cuttings, John M. Ruter

Adventitious Rooting of Woody and Herbaceous Plants, Lori D. Osburn, Zong-Ming Cheng, and Robert N. Trigiano

Long Cuttings: A Novel Method to Propagate Woody Plants, Wolfgang Spethmann

Rooting Cuttings of Tropical Plants, Richard A. Criley


Adventitious Shoot and Root Formation on Leaf and Root Cuttings, Caula A. Beyl

Propagation by Leaf Cuttings, John L. Griffis, Jr. and Malcom M. Manners

Propagation by Root Cuttings, Paul E. Read


Layering, Brian Maynard


Grafting: Theory and Practice, Kenneth W. Mudge

Grafting and Budding with Woody and Herbaceous Species, Garry V. McDonald

Grafting with Vegetable Plants, Chieri Kubota

Bulbs and Plants with Special Structures

Storage Organs, Jeffrey A. Adkins and William B. Miller

Propagating Selected Flower Bulb Species, William B. Miller, Jeffrey A. Adkins, and John E. Preece


Micropropagation, Michael E. Kane, Philip J. Kauth, and Scott L. Stewart

Getting Started with Tissue Culture: Media Preparation, Sterile Technique, and Laboratory Equipment, Caula A. Beyl

Micropropagation of Mint, Sherry L. Kitto

Micropropagation of Tropical Root and Tuber Crops, Leopold M. Nyochembeng

Micropropagation of Woody Plants, Robert R. Tripepi


Seed Production, Processing and Analysis, J. Kim Pittcock

Practices to Promote Seed Germination, Caula A. Beyl

Producing Seedlings and Bedding Plants, Holly L. Scoggins


Myths of Plant Propagation, Jeffrey H. Gillman

Intellectual Property Protection for Plants, Christopher Eisenschenk

Molecular Biology and the Future of Plant Propagation, Govind C. Sharma, Manan Sharma, and Caula A. Beyl


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Caula A. Beyl is the dean of the College of Agricultural Sciences and Natural Resources at the University of Tennessee. She obtained a master’s degree in horticulture and a PhD in the area of stress physiology from Purdue University. In more than 34 years as a researcher, teacher, and administrator, she has served as a principal investigator or co-investigator on 42 funded research projects in various areas of horticulture, stress physiology, and space biology. Dr. Beyl has been elected as a fellow in the American Society for Horticultural Science and also received the School of Agriculture Outstanding Researcher award at Alabama A&M University. In 2008, she received the Outstanding College of Agriculture Alumna award from Purdue University.

Robert N. Trigiano is a professor at the Department of Entomology and Plant Pathology at the University of Tennessee. He received a master’s degree in biology from Pennsylvania State University and a PhD in botany and plant pathology from North Carolina State University at Raleigh. Dr. Trigiano is a member of the American Phytopathological Society and the American Society for Horticultural Science. He was elected Fellow of the American Society of Horticultural Science and has published more than 200 research papers, book chapters, patents, and popular press articles.


"For the last six years, I have been searching for a laboratory exercise book on plant propagation to complement my class textbook. The laboratories in the course are designed to help students to develop a wide range of propagation techniques and understand the underlying principles. However, I often was asked by my students how to set up a small propagation research trial independently. This new revision of propagation exercises edited by Caula Beyl and Robert Trigiano fits my needs...

"...There are unique features that distinguish this brook from other plant propagation books. It includes a wide range of vital information related to plant propagation on topics from foundation of plant science, theory, and concept to laboratory exercises. This book provides not only an in-depth presentation of its subject but also laboratory exercises aligned well with the topic of each chapter. While encompassing every topic that is necessary with plant propagation, the laboratory exercises provide in-depth supplementary information and hands-on practices that represent the principles in the chapters. Particularly, it has a list of materials necessary to complete the experiments, step-bystep guidelines that are easy to follow through for beginners, examples of data collection, anticipated results of the laboratory exercises, and a list of critical questions. This book contributes to plant propagation learning and education by providing essential information in a clear, concise, and attentive manner, by cultivating a self-directed learning environment, by including plant materials from temperate to tropical regions, and by introducing past, current, and future techniques. Overall, this book is an excellent resource for a college, plant propagation course, either as a supportive reading material for a higher-level propagation course or as a single textbook for a lower-level course. It will be invaluable for students to enhance their skills and develop confidence in themselves as independet learners."

Department of Horticulture and Landscape
Purdue University
West Lafayette, Indiana